Özmen Mehmet Ferit, Cirit Ümüt, Arıcı Ramazan, Demir Kamber, Kurt Doğan, Pabuccuoğlu Serhat, Ak Kemal
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Ceyhan Veterinary Medicine, Cukurova University, Adana, Turkey.
Andrologia. 2020 Oct;52(9):e13656. doi: 10.1111/and.13656. Epub 2020 Jun 1.
The primary aim of the study was to investigate whether iodixanol and trehalose would have a synergic effect on the cryosurvival of electroejaculated ram semen. Tris-based diluter was used to prepare 9 different extenders by the addition of iodixanol or trehalose alone or varying combinations of these substances. Diluters were prepared as follows: Tris (control), Io5 (5% iodixanol), Tr25 (25 mmol/L trehalose), Tr50 (50 mmol/L trehalose), Tr50 + Io1.25 (50 mmol/L trehalose and 1.25% iodixanol), Tr50 + Io2.5 (50 mmol/L trehalose and 2.5% iodixanol), Tr50 + Io5 (50 mmol/L trehalose and 5% iodixanol), Tr25 + Io5 (25 mmol/L trehalose and 5% iodixanol) and Tr12.5 + Io5 (12.5 mmol/L trehalose and 5% iodixanol). Supplementation of the freezing extender with trehalose or iodixanol alone supported the protection of both morphological and functional integrity of ram spermatozoa and total motility at 1 and 4 hr post-thawing respectively. However, beyond these positive effects, the combination of trehalose (25 mmol/L) and iodixanol (5%) significantly increased post-thaw sperm longevity and motion properties at the end of 4-hr incubation. The results of the study clearly showed that there was positive synergic effect of iodixanol and trehalose on cryosurvival of ram semen.
本研究的主要目的是调查碘克沙醇和海藻糖对电刺激采精的公羊精液冷冻存活是否具有协同作用。使用基于Tris的稀释剂，通过单独添加碘克沙醇或海藻糖或这些物质的不同组合来制备9种不同的稀释液。稀释液的制备方法如下：Tris（对照）、Io5（5%碘克沙醇）、Tr25（25 mmol/L海藻糖）、Tr50（50 mmol/L海藻糖）、Tr50 + Io1.25（50 mmol/L海藻糖和1.25%碘克沙醇）、Tr50 + Io2.5（50 mmol/L海藻糖和2.5%碘克沙醇）、Tr50 + Io5（50 mmol/L海藻糖和5%碘克沙醇）、Tr25 + Io5（25 mmol/L海藻糖和5%碘克沙醇）以及Tr12.5 + Io5（12.5 mmol/L海藻糖和5%碘克沙醇）。单独用海藻糖或碘克沙醇补充冷冻稀释液分别在解冻后1小时和4小时时支持保护公羊精子的形态和功能完整性以及总活力。然而，除了这些积极作用外，海藻糖（25 mmol/L）和碘克沙醇（5%）的组合在4小时孵育结束时显著提高了解冻后精子的寿命和运动特性。该研究结果清楚地表明，碘克沙醇和海藻糖对公羊精液的冷冻存活具有积极的协同作用。
