State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200438, China.
ACS Appl Mater Interfaces. 2020 Jun 24;12(25):27971-27983. doi: 10.1021/acsami.0c05024. Epub 2020 Jun 12.
Cell migration on material surfaces is a fundament issue in the fields of biomaterials, cell biology, tissue engineering, regenerative medicine, etc. Herein, we aim to guide cell migration by flat microstripes with significant contrast of cell adhesion and varied geometric features of the adhesive stripes. To this end, we designed and fabricated cell-adhesive arginine-glycine-aspartate (RGD) microstripes on the nonfouling poly(ethylene glycol) (PEG) background and examined the microstripe-guided adhesion and migration of a few cell types. The migration of cell clusters adhering on the RGD regions was found to be significantly affected by the widths and arc radiuses of the guided microstripes. The cells migrated fastest on the straight microstripes with width of about 20 μm, which we defined as single file confined migration (SFCM). We also checked the possible left-right asymmetric bias of cell migration guided by combinatory microstripes with alternative wavy and quasi-straight stripes under a given width, and found that the velocity of CCW (counter clockwise) migration was higher than that of CW (clockwise) migration for primary rat mesenchymal stem cells (rMSCs), whereas no left-right asymmetric bias was observed for NIH3T3 (mouse embryonic fibroblast cell line) and Hela (human cervix epithelial carcinoma cell line) cells. Comparison of migration of cells on the nanotopological stripe and smooth surfaces further confirmed the importance of cell orientation coherence for guided cell migration and strengthened the superiority of SFCM.
细胞在材料表面的迁移是生物材料学、细胞生物学、组织工程学、再生医学等领域的基础问题。在此，我们旨在通过具有显著细胞黏附差异和不同黏附条纹几何特征的平微条纹来引导细胞迁移。为此，我们在非黏附聚乙二醇（PEG）基底上设计并制作了细胞黏附的精氨酸-甘氨酸-天冬氨酸（RGD）微条纹，并研究了几种细胞类型在微条纹引导下的黏附和迁移。发现黏附在 RGD 区域的细胞簇的迁移明显受到导向微条纹的宽度和圆弧半径的影响。细胞在宽度约为 20μm 的直微条纹上迁移最快，我们将其定义为单排受限迁移（SFCM）。我们还检查了在给定宽度下，具有交替波浪形和准直条纹的组合微条纹引导的细胞迁移可能存在的左右不对称性，并发现原代大鼠间充质干细胞（rMSCs）的逆时针（CCW）迁移速度高于顺时针（CW）迁移速度，而 NIH3T3（小鼠胚胎成纤维细胞系）和 Hela（人宫颈上皮癌细胞系）细胞则没有观察到左右不对称性。细胞在纳米拓扑条纹和光滑表面上的迁移比较进一步证实了细胞取向一致性对导向细胞迁移的重要性，并强化了 SFCM 的优势。
