State Key Laboratory of Molecular Engineering of Polymers; Department of Macromolecular Science; Fudan University; Shanghai, PR China.
Organogenesis. 2013 Oct 1;9(4):280-6. doi: 10.4161/org.26080. Epub 2013 Aug 19.
The present report is an extension of our preceding publication in Biomaterials (2013) entitled "Effect of RGD nanospacing on differentiation of stem cells." Cell-adhesive peptide arginine-glycine-aspartate (RGD) was nanopatterned on a non-fouling poly(ethylene glycol) (PEG) hydrogel, and mesenchymal stem cells (MSCs) derived from rat bone marrow were cultured on the patterned surfaces at nanospacings from 37 to 124 nm. Cell adhesion parameters such as spreading areas varied with RGD nanospacings significantly. The differences were well observed at both the first and eighth days, which confirmed the persistence of this nanospacing effect on our nanopatterns. The proliferation rate also varied with the nanospacings. Osteogenic and adipogenic inductions were undertaken, and a significant influence of RGD nanospacing on stem cell differentiation was found. The effect on differentiation cannot be simply interpreted by differences in cell adhesion and proliferation. We further calculated the fractions of single, coupled, and multiple cells on those nanopatterns, and ruled out the possibility that the extent of cell-cell contact determined the different differentiation fractions. Accordingly, we reinforced the idea that RGD nanospacing might directly influence stem cell differentiation.
本报告是对我们之前在《生物材料》（2013 年）发表的题为“RGD 纳米间距对干细胞分化的影响”一文的扩展。细胞黏附肽精氨酸-甘氨酸-天冬氨酸（RGD）被图案化在非固定聚乙二醇（PEG）水凝胶上，并且源自大鼠骨髓的间充质干细胞（MSCs）在图案化表面上以 37 至 124nm 的纳米间距培养。细胞黏附参数，如扩展面积随 RGD 纳米间距显著变化。在第 1 天和第 8 天都能很好地观察到差异，这证实了我们的纳米图案上存在这种纳米间距效应的持久性。增殖率也随纳米间距而变化。进行了成骨和脂肪诱导，发现 RGD 纳米间距对干细胞分化有显著影响。这种对分化的影响不能简单地用细胞黏附和增殖的差异来解释。我们进一步计算了那些纳米图案上单细胞、偶联细胞和多细胞的分数，并排除了细胞-细胞接触程度决定不同分化分数的可能性。因此，我们强化了 RGD 纳米间距可能直接影响干细胞分化的观点。
