Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Av. do Café s/n, Monte Alegre, Ribeirão Preto - SP, 14040-903, Brazil.
Companhia Ambiental do Estado de São Paulo (CETESB), Ribeirão Preto, Brazil.
Int J Antimicrob Agents. 2020 Jul;56(1):106040. doi: 10.1016/j.ijantimicag.2020.106040. Epub 2020 May 29.
Aquatic environments are considered a reservoir for the dissemination of multidrug-resistant (MDR) bacteria, principally Escherichia coli, with the consequent spread of acquired antimicrobial resistance genes (ARGs). Widespread high-risk clones of MDR E. coli are responsible for human infections worldwide. This study aimed to characterise, through whole-genome sequencing (WGS), isolates of MDR E. coli harbouring ARGs obtained from public aquatic environments in Brazil. MDR E. coli isolates were obtained from rivers, streams and lakes that presented different Water Quality Index records and were submitted to WGS. The resistome, mobilome and virulome showed a great diversity of ARGs, plasmids and virulence genes, respectively. In addition, mutations in the quinolone resistance-determining regions of GyrA, ParC and ParE as well as several metal resistance genes (MRGs) and antibacterial biocide resistance genes (ABGs) were detected. Typing and subtyping of MDR E. coli revealed different lineages, with two belonging to widespread high-risk clones (i.e. B2-ST131-fimH30 and F-ST648-fimH27), which are grouped by core genome multilocus sequence typing (cgMLST) in clusters with E. coli lineages obtained from different sources distributed worldwide. MDR bacteria carrying MRGs and ABGs have emerged as a global human and environmental health problem. Detection of widespread high-risk clones calls for attention to the dissemination of fluoroquinolone-resistant QnrS1- and CTX-M-producing E. coli lineages associated with human infections in public aquatic environments.
水生环境被认为是传播多药耐药（MDR）细菌的储库，主要是大肠杆菌，随之而来的是获得性抗菌耐药基因（ARGs）的传播。广泛存在的高风险 MDR 大肠杆菌克隆株负责世界范围内的人类感染。本研究旨在通过全基因组测序（WGS）对巴西公共水生环境中获得的携带 ARGs 的 MDR 大肠杆菌进行表征。从具有不同水质指数记录的河流、溪流和湖泊中获得 MDR 大肠杆菌分离株，并进行 WGS。耐药组、可移动组和毒力组分别显示出 ARGs、质粒和毒力基因的多样性。此外，还检测到喹诺酮类药物耐药决定区 GyrA、ParC 和 ParE 中的突变以及几种金属耐药基因（MRGs）和抗菌杀生物剂耐药基因（ABGs）。MDR 大肠杆菌的分型和亚型显示出不同的谱系，其中两个属于广泛存在的高风险克隆株（即 B2-ST131-fimH30 和 F-ST648-fimH27），它们通过核心基因组多位点序列分型（cgMLST）聚类与来自世界各地不同来源的大肠杆菌谱系分组。携带 MRGs 和 ABGs 的 MDR 细菌已成为全球人类和环境健康问题。广泛存在的高风险克隆株的检测需要注意传播与人类感染相关的氟喹诺酮类耐药 QnrS1-和 CTX-M 产生大肠杆菌谱系。
