Motta C, Palcoux J B, Meyer M, Roche M
Laboratoire de Biochimie, Hôtel-Dieu, Clermont-Ferrand.
Ann Med Interne (Paris). 1988;139 Suppl 1:83-5.
Two young girls with type IIa homozygotic familial hypercholesterolemia were treated by plasma exchange to reduce circulating cholesterol levels, values prior to treatment being abnormally elevated (greater than 10 mmol/l). Simultaneous determination of membrane fluidity (by fluorescence polarization) of erythrocytes and lymphocytes showed marked decreases due principally to an increase in the intramembrane cholesterol/phospholipid ratio. Values after treatment showed a tendency to return to physiologic levels without, however, attaining normal values. In vitro studies of cholesterol incorporation in these membranes demonstrated the primordial role of cholesterol in these processes of membrane rigidity, and their reversibility. It is suggested that certain physical membranal properties could provide a useful index to assess need for more or less frequent plasma exchanges.
两名患有IIa型纯合子家族性高胆固醇血症的年轻女孩接受了血浆置换治疗，以降低循环胆固醇水平，治疗前该值异常升高（大于10 mmol/l）。同时测定红细胞和淋巴细胞的膜流动性（通过荧光偏振）显示明显降低，主要原因是膜内胆固醇/磷脂比率增加。治疗后的数值显示有恢复到生理水平的趋势，然而并未达到正常值。对这些膜中胆固醇掺入的体外研究证明了胆固醇在这些膜刚性过程及其可逆性中的首要作用。有人提出，某些膜的物理性质可为评估更频繁或较不频繁进行血浆置换的必要性提供有用指标。
