通过聚乙亚胺沉淀法纯化限制性内切酶EcoRI。 - Suppr

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The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine.

作者信息

Bingham A H, Sharman A F, Atkinson T

出版信息

FEBS Lett. 1977 Apr 15;76(2):250-6. doi: 10.1016/0014-5793(77)80162-2.

DOI:10.1016/0014-5793(77)80162-2

PMID:324801

Abstract

摘要

相似文献

The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine.通过聚乙亚胺沉淀法纯化限制性内切酶EcoRI。

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[Construction of recombinant DNA molecules in vitro and cloning by using EcoRI the activity of restriction endonuclease EcoRI].[体外重组DNA分子的构建及利用EcoRI（限制性内切核酸酶EcoRI的活性）进行克隆]

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Solubility of the EcoRI restriction endonuclease and its purification from an over-producing strain.EcoRI 限制性内切酶的溶解性及其从高产菌株中的纯化。

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Precipitation of nucleic acids with polyethyleneimine and the chromatography of nucleic acids and proteins on immobilised polyethyleneimine.用聚乙烯亚胺沉淀核酸以及核酸和蛋白质在固定化聚乙烯亚胺上的色谱分析

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引用本文的文献

Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro.基因6核酸外切酶缺陷的噬菌体T7在体内和体外均能促进T7 DNA的位点特异性切割。

J Virol. 1982 Oct;44(1):235-40. doi: 10.1128/JVI.44.1.235-240.1982.

Genetic recombination of bacteriophage T7 in vivo studied by use of a simple physical assay.利用一种简单的物理检测方法对噬菌体T7在体内的基因重组进行研究。

J Virol. 1981 Dec;40(3):839-47. doi: 10.1128/JVI.40.3.839-847.1981.

Non-specific binding of restriction endonuclease EcoR1 to DNA.限制性内切酶EcoR1与DNA的非特异性结合。

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Nuclear RNA transcripts from Drosophila melanogaster ribosomal RNA genes containing introns.来自含有内含子的黑腹果蝇核糖体RNA基因的核RNA转录本。

Nucleic Acids Res. 1980 Jan 11;8(1):67-84. doi: 10.1093/nar/8.1.67.

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EMBO J. 1982;1(1):107-14. doi: 10.1002/j.1460-2075.1982.tb01132.x.

A simple, general procedure for purifying restriction endonucleases.一种纯化限制性核酸内切酶的简单通用方法。

Nucleic Acids Res. 1977 Aug;4(8):2561-72. doi: 10.1093/nar/4.8.2561.

Structure of yeast phenylalanine-tRNA genes: an intervening DNA segment within the region coding for the tRNA.酵母苯丙氨酸 - tRNA基因的结构：编码tRNA区域内的一段间隔DNA序列。

Proc Natl Acad Sci U S A. 1978 Jan;75(1):190-4. doi: 10.1073/pnas.75.1.190.

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