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基因6核酸外切酶缺陷的噬菌体T7在体内和体外均能促进T7 DNA的位点特异性切割。

Bacteriophage T7 defective in the gene 6 exonuclease promotes site-specific cleavages of T7 DNA in vivo and in vitro.

作者信息

Lee D D, Sadowski P D

出版信息

J Virol. 1982 Oct;44(1):235-40. doi: 10.1128/JVI.44.1.235-240.1982.

DOI:10.1128/JVI.44.1.235-240.1982
PMID:7143567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256257/
Abstract

Site-specific cleavages of intracellular DNA were demonstrated in bacteriophage T7 6am-infected cells. The sites of the cleavages were located at 46.8 and 68.7% (1% of the T7 DNA length = 400 base pairs) from the left end of the T7 genome. These cleavages required the products of genes 3 (endonuclease), 4 (DNA primase), and 5 (DNA polymerase). However, the product of gene 6 (exonuclease) must be absent. Site-specific cleavage was also shown to occur in vitro in extracts of T7 6am-infected cells, although at a different site: 82.8% from the left end of the T7 genome.

摘要

在噬菌体T7 6am感染的细胞中证实了细胞内DNA的位点特异性切割。切割位点位于T7基因组左端的46.8%和68.7%处（T7 DNA长度的1% = 400个碱基对）。这些切割需要基因3（核酸内切酶）、基因4（DNA引发酶）和基因5（DNA聚合酶）的产物。然而，基因6（核酸外切酶）的产物必须不存在。位点特异性切割也在T7 6am感染细胞的提取物中体外发生，尽管在不同位点：位于T7基因组左端的82.8%处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/2e6c3eee2866/jvirol00151-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/085ade0ea10d/jvirol00151-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/de960a8816dc/jvirol00151-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/61c086a01aef/jvirol00151-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/0e825b45e69b/jvirol00151-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/2e6c3eee2866/jvirol00151-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/085ade0ea10d/jvirol00151-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/de960a8816dc/jvirol00151-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/61c086a01aef/jvirol00151-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/0e825b45e69b/jvirol00151-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b531/256257/2e6c3eee2866/jvirol00151-0249-a.jpg

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引用本文的文献

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本文引用的文献

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Cruciform structures in supercoiled DNA.超螺旋DNA中的十字形结构。
Nature. 1981 Feb 5;289(5797):466-70. doi: 10.1038/289466a0.
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Genetic and physical mapping of the late region of bacteriophage T7 DNA by use of cloned fragments of T7 DNA.利用噬菌体T7 DNA的克隆片段对噬菌体T7 DNA晚期区域进行遗传和物理图谱分析。
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Degradation of Escherichia coli B deoxyribonucleic acid after infection with deoxyribonucleic acid-defective amber mutants of bacteriophage T7.用噬菌体T7的脱氧核糖核酸缺陷型琥珀突变体感染后大肠杆菌B脱氧核糖核酸的降解
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An endonuclease induced after infection of Escherichia coli with bacteriophage T7. I. Purification and properties of the enzyme.噬菌体T7感染大肠杆菌后诱导产生的一种核酸内切酶。I. 该酶的纯化及性质
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