Köhl Karin, Tohge Takayuki, Schöttler Mark Aurel
Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany.
Funct Plant Biol. 2017 Jun;44(7):727-738. doi: 10.1071/FP17051.
For precise phenotyping, Arabidopsis thaliana (L.) Heynh. is grown under controlled conditions with fluorescent lamps as the predominant light source. Replacement by systems based on light emitting diodes (LED) could improve energy efficiency and stability of light quality and intensity. To determine whether this affects the reproducibility of results obtained under fluorescent lamps, four Arabidopsis accessions and a phytochrome mutant were grown and phenotyped under two different LED types or under fluorescent lamps. All genotypes had significantly higher rosette weight and seed mass and developed faster under LED light than under fluorescent lamps. However, differences between genotypes were reproducible independent of the light source. Chlorophyll content, photosynthetic complex accumulation and light response curves of chlorophyll fluorescence parameters were indistinguishable under LED and fluorescent light. Principal component analysis of leaf metabolite concentrations revealed that the effect of a change from fluorescent light to LED light was small compared with the diurnal effect, which explains 74% of the variance and the age effect during vegetative growth (12%). Altogether, the replacement of fluorescent lamps by LED allowed Arabidopsis cultivation and reproduction of results obtained under fluorescent light.
为了进行精确的表型分析，拟南芥（Arabidopsis thaliana (L.) Heynh.）在以荧光灯作为主要光源的可控条件下生长。用基于发光二极管（LED）的系统替代荧光灯可提高能源效率以及光质和光强的稳定性。为了确定这是否会影响在荧光灯下获得的结果的可重复性，在两种不同类型的LED灯下或荧光灯下种植并对四种拟南芥种质和一个光敏色素突变体进行了表型分析。与荧光灯下相比，所有基因型在LED灯下莲座叶重量和种子质量均显著更高，且发育更快。然而，基因型之间的差异与光源无关，是可重复的。在LED光和荧光灯下，叶绿素含量、光合复合体积累以及叶绿素荧光参数的光响应曲线没有差异。对叶片代谢物浓度进行主成分分析发现，与昼夜效应相比，从荧光灯换成LED灯的影响较小，昼夜效应解释了74%的变异，而营养生长期间的年龄效应解释了12%的变异。总体而言，用LED灯替代荧光灯能够实现拟南芥的种植，并重现荧光灯下获得的结果。
