Abulateefeh Samer R, Spain Sebastian G, Thurecht Kristofer J, Aylott Jonathan W, Chan Weng C, Garnett Martin C, Alexander Cameron
School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK.
Biomater Sci. 2013 Apr 5;1(4):434-442. doi: 10.1039/c2bm00184e. Epub 2013 Jan 14.
Polymer particles consisting of a biodegradable poly[lactide-co-glycolide] (PLGA) core and a thermoresponsive shell have been formulated to encapsulate the dye rhodamine 6G and the potent cytotoxic drug paclitaxel. Cellular uptake of these particles is significantly enhanced above the thermal transition temperature (TTT) of the polymer shells in the human breast carcinoma cell line MCF-7 as determined by flow cytometry and fluorescence microscopy. Paclitaxel-loaded particles display reduced and enhanced cytotoxicity below and above the TTT respectively compared to unencapsulated drug. The data suggests a potential route to enhanced anti-cancer efficacy through temperature-mediated cell targeting.
由可生物降解的聚丙交酯-乙交酯共聚物（PLGA）核和热响应性壳组成的聚合物颗粒已被制备用于包封染料罗丹明6G和强效细胞毒性药物紫杉醇。通过流式细胞术和荧光显微镜测定，在人乳腺癌细胞系MCF-7中，这些颗粒在聚合物壳的热转变温度（TTT）以上的细胞摄取显著增强。与未包封的药物相比，负载紫杉醇的颗粒在TTT以下和以上分别显示出降低和增强的细胞毒性。数据表明通过温度介导的细胞靶向提高抗癌疗效的潜在途径。
