Jang Myung-Soo, Han Jin Hee, Kim Dong-Ok, Woo Gil, Kwon Jae-Ik, Chung Jun-Young, Lee Bong-Jae, Yi Jae-Woo
Department of Anesthesiology and Pain Medicine, Kyung Hee University Medical Center, College of Medicine, Kyung Hee University, Seoul, Korea.
Department of Anesthesiology and Pain Medicine, Kyung Hee University Hospital at Gangdong, College of Medicine, Kyung Hee University, Seoul, Korea.
Int Neurourol J. 2020 May;24(Suppl 1):S11-18. doi: 10.5213/inj.2040162.081. Epub 2020 May 31.
The effects of dexmedetomidine on locomotor function and thermal hyperalgesia in sciatic nerve crush injury (SNCI) were investigated using rats.
After exposing the right sciatic nerve, the sciatic nerve was crushed for 1 minute by a surgical clip. One day after nerve injury, dexmedetomidine (5, 25, and 50 µg/kg) was directly applied to the injured sciatic nerve once a day for 14 days. Walking track analysis was used to assess locomotor function and plantar test was conducted to assess thermal pain sensitivity. Immunohistochemistry was performed to determine the expression of c-Fos in the ventrolateral periaqueductal gray (vlPAG) and paraventricular nucleus (PVN). Western blot was used to evaluate the expression level of nerve growth factor (NGF) and myelin basic protein (MBP) in the sciatic nerve.
SNCI resulted in deterioration of locomotor function and increased thermal pain sensitivity. The level of c-Fos expression in the PVN and vlPAG was increased and the level of NGF and MBP expression in the sciatic nerve was enhanced by SNCI. Dexmedetomidine treatment improved locomotor function and upregulated expression of NGF and MBP in the sciatic nerve of SNCI. Dexmedetomidine treatment alleviated thermal hyperalgesia and downregulated expression of c-Fos in the vlPAG and PVN after SNCI.
Dexmedetomidine may be used as a potential new treatment drug for recovery of locomotion and control of pain in peripheral nerve injury.
利用大鼠研究右美托咪定对坐骨神经挤压伤（SNCI）后运动功能和热痛觉过敏的影响。
暴露右侧坐骨神经后，用手术夹将坐骨神经挤压1分钟。神经损伤1天后，右美托咪定（5、25和50μg/kg）每天直接应用于损伤的坐骨神经，持续14天。采用行走轨迹分析评估运动功能，并进行足底试验评估热痛敏感性。进行免疫组织化学检测以确定腹外侧导水管周围灰质（vlPAG）和室旁核（PVN）中c-Fos的表达。采用蛋白质免疫印迹法评估坐骨神经中神经生长因子（NGF）和髓鞘碱性蛋白（MBP）的表达水平。
SNCI导致运动功能恶化和热痛敏感性增加。SNCI使PVN和vlPAG中c-Fos表达水平升高，坐骨神经中NGF和MBP表达水平增强。右美托咪定治疗改善了SNCI大鼠的运动功能，并上调了坐骨神经中NGF和MBP的表达。右美托咪定治疗减轻了热痛觉过敏，并下调了SNCI后vlPAG和PVN中c-Fos的表达。
右美托咪定可能作为一种潜在的新治疗药物用于外周神经损伤后运动恢复和疼痛控制。
