Beigi Mohammad-Hossein, Ghasemi-Mobarakeh Laleh, Prabhakaran Molamma P, Karbalaie Khadijeh, Azadeh Hamid, Ramakrishna Seeram, Baharvand Hossein, Nasr-Esfahani Mohammad-Hossein
Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Materials Engineering Department, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
J Biomed Mater Res A. 2014 Dec;102(12):4554-67. doi: 10.1002/jbm.a.35119. Epub 2014 Mar 3.
Artificial nanofiber nerve guides have gained huge interest in bridging nerve gaps and associated peripheral nerve regeneration due to its high surface area, flexibility and porous structure. In this study, electrospun poly (ε-caprolactone)/gelatin (PCL/Gel) nanofibrous mats were fabricated, rolled around a copper wire and fixed by medical grade adhesive to obtain a tubular shaped bio-graft, to bridge 10 mm sciatic nerve gap in in vivo rat models. Stem cells from human exfoliated deciduous tooth (SHED) were transplanted to the site of nerve injury through the nanofibrous nerve guides. In vivo experiments were performed in animal models after creating a sciatic nerve gap, such that the nerve gap was grafted using (i) nanofiber nerve guide (ii) nanofiber nerve guide seeded with SHED (iii) suturing, while an untreated nerve gap remained as the negative control. In vitro cell culture study was carried out for primary investigation of SHED-nanofiber interaction and its viability within the nerve guides after 2 and 16 weeks of implantation time. Walking track analysis, plantar test, electrophysiology and immunohistochemistry were performed to evaluate functional recovery during nerve regeneration. Vascularization was also investigated by hematoxilin/eosine (H&E) staining. Overall results showed that the SHED seeded on nanofibrous nerve guide could survive and promote axonal regeneration in rat sciatic nerves, whereby the biocompatible PCL/Gel nerve guide with cells can support axonal regeneration and could be a promising tissue engineered graft for peripheral nerve regeneration.
人工纳米纤维神经导管因其高表面积、柔韧性和多孔结构，在桥接神经间隙及相关周围神经再生方面引起了广泛关注。在本研究中，制备了电纺聚（ε-己内酯）/明胶（PCL/Gel）纳米纤维垫，将其缠绕在铜丝上并用医用级粘合剂固定，以获得管状生物移植物，用于在体内大鼠模型中桥接10mm的坐骨神经间隙。将人脱落乳牙干细胞（SHED）通过纳米纤维神经导管移植到神经损伤部位。在建立坐骨神经间隙后，在动物模型中进行体内实验，使神经间隙分别用（i）纳米纤维神经导管、（ii）接种SHED的纳米纤维神经导管、（iii）缝合进行移植，同时将未处理的神经间隙作为阴性对照。进行体外细胞培养研究，初步探究植入2周和16周后SHED与纳米纤维的相互作用及其在神经导管内的活力。通过行走轨迹分析、足底试验、电生理学和免疫组织化学来评估神经再生过程中的功能恢复情况。还通过苏木精/伊红（H&E）染色研究血管化情况。总体结果表明，接种在纳米纤维神经导管上的SHED能够存活并促进大鼠坐骨神经的轴突再生，由此，具有生物相容性的含细胞PCL/Gel神经导管能够支持轴突再生，可能是一种有前景的用于周围神经再生的组织工程移植物。
