Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Shanghai Public Health Clinical Center Fudan University, Shanghai, China.
Integrated Mycobacterial Pathogenomics Unit, Institut Pasteur, Paris, France.
Antimicrob Agents Chemother. 2020 Jul 22;64(8). doi: 10.1128/AAC.00224-20.
We isolated spontaneous levofloxacin-resistant strains of to study the fitness cost and compensatory evolution of fluoroquinolone resistance in mycobacteria. Five of six mutant strains with substantial growth defects showed restored fitness after being serially passaged for 18 growth cycles, along with increased cellular ATP level. Whole-genome sequencing identified putative compensatory mutations in the gene that restored the fitness of the resistant strains, presumably by altering the bacterial energy metabolism.
我们分离了结核分枝杆菌自发左氧氟沙星耐药株，以研究氟喹诺酮类耐药在分枝杆菌中的适应性代价和补偿进化。6 株突变株中有 5 株生长缺陷明显，经过 18 个传代生长周期后，细胞 ATP 水平升高，恢复了生长能力。全基因组测序鉴定了可能的补偿性突变，这些突变发生在 基因，可能通过改变细菌的能量代谢来恢复耐药株的生长能力。
