Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R. China.
Genomics and Bioinformatics Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar 25000 Khyber Pakhtunkhwa, Pakistan.
J Microbiol Biotechnol. 2020 May 28;30(5):668-680. doi: 10.4014/jmb.1910.10021.
is an important plant growth-promoting rhizobacterium with immense potential in agriculture development. In the present study, Lle-9 was isolated from the bulbs of . The isolated strain showed antifungal activities against plant pathogens like , , and . The highest percentage of growth inhibition , 68.56±2.35% was observed against followed by 63.12 ± 2.83%, 61.67 ± 3.39% and 55.82 ± 2.76% against , , and , respectively. The ethyl acetate fraction revealed a number of bioactive compounds and several were identified as antimicrobial agents such as diketopiperazines, cyclo-peptides, linear peptides, latrunculin A, 5α-hydroxy-6-ketocholesterol, (R)-S-lactoylglutathione, triamterene, rubiadin, moxifloxacin, 9-hydroxy-5Z,7E,11Z,14Zeicosatetraenoic acid, D-erythro-C18-Sphingosine, citrinin, and 2- arachidonoyllysophosphatidylcholine. The presence of these antimicrobial compounds in the bacterial culture might have contributed to the antifungal activities of the isolated Lle- 9. The strain showed plant growth-promoting traits such as production of organic acids, ACC deaminase, indole-3-acetic acid (IAA), siderophores, and nitrogen fixation and phosphate solubilization. IAA production was accelerated with application of exogenous tryptophan concentrations in the medium. Further, the lily plants upon inoculation with Lle-9 exhibited improved vegetative growth, more flowering shoots and longer roots than control plants under greenhouse condition. The isolated strain Lle-9 possessed broad-spectrum antifungal activities and multiple plant growth-promoting traits and thus may play an important role in promoting sustainable agriculture. This strain could be developed and applied in field experiments in order to promote plant growth and control disease pathogens.
是一种重要的植物促生根际细菌，在农业发展中具有巨大的潜力。本研究从百合鳞茎中分离得到 Lle-9 菌株。该分离菌株对植物病原菌如 、 、 和 具有抗真菌活性。观察到对 的生长抑制率最高，为 68.56±2.35%，其次是对 、 、 和 的 63.12 ± 2.83%、61.67 ± 3.39%和 55.82 ± 2.76%。乙酸乙酯部分显示出许多生物活性化合物，其中一些被鉴定为抗菌剂，如二酮哌嗪、环肽、线性肽、拉他丁 A、5α-羟基-6-酮胆固醇、(R)-S-乳酰谷胱甘肽、三甲氨、红紫素、莫西沙星、9-羟基-5Z,7E,11Z,14Z 二十碳四烯酸、D-erythro-C18-Sphingosine、桔霉素和 2-花生四烯酰基溶血磷脂酰胆碱。这些抗菌化合物在细菌培养物中的存在可能是分离的 Lle-9 菌株抗真菌活性的原因。该菌株表现出植物促生特性，如产生有机酸、ACC 脱氨酶、吲哚-3-乙酸（IAA）、铁载体和固氮以及磷溶解。在培养基中添加外源性色氨酸浓度可加速 IAA 的产生。此外，在温室条件下，接种 Lle-9 的百合植物表现出比对照植物更好的营养生长、更多的开花枝和更长的根。分离的 菌株 Lle-9 具有广谱抗真菌活性和多种植物促生特性，因此可能在促进可持续农业方面发挥重要作用。该菌株可以开发并应用于田间试验，以促进植物生长和控制病害病原体。
