Sitnikov Dmitry S, Ilina Inna V, Revkova Veronika A, Rodionov Sergey A, Gurova Svetlana A, Shatalova Rimma O, Kovalev Alexey V, Ovchinnikov Andrey V, Chefonov Oleg V, Konoplyannikov Mikhail A, Kalsin Vladimir A, Baklaushev Vladimir P
Joint Institute for High Temperatures of the Russian Academy of Sciences, Moscow, Russia.
Federal Research and Clinical Center of Specialized Medical Care and Medical Technologies FMBA of Russia, Moscow, Russia.
Biomed Opt Express. 2021 Oct 26;12(11):7122-7138. doi: 10.1364/BOE.440460. eCollection 2021 Nov 1.
For the first time, the data have been obtained on the effects of high-intensity terahertz (THz) radiation (with the intensity of 30 GW/cm, electric field strength of 3.5 MV/cm) on human skin fibroblasts. A quantitative estimation of the number of histone Н2АХ foci of phosphorylation was performed. The number of foci per cell was studied depending on the irradiation time, as well as on the THz pulse energy. The performed studies have shown that the appearance of the foci is not related to either the oxidative stress (the cells preserve their morphology, cytoskeleton structure, and the reactive oxygen species content does not exceed the control values), or the thermal effect of THz radiation. The prolonged irradiation of fibroblasts also did not result in a decrease of their proliferative index.
首次获得了关于高强度太赫兹（THz）辐射（强度为30 GW/cm，电场强度为3.5 MV/cm）对人皮肤成纤维细胞影响的数据。对组蛋白H2AX磷酸化位点的数量进行了定量估计。研究了每个细胞中位点的数量与照射时间以及太赫兹脉冲能量的关系。所进行的研究表明，这些位点的出现既与氧化应激无关（细胞保持其形态、细胞骨架结构，活性氧含量不超过对照值），也与太赫兹辐射的热效应无关。成纤维细胞的长时间照射也未导致其增殖指数下降。
