Gould Richard, McFadden Sonyia L, Horn Simon, Prise Kevin M, Doyle Philip, Hughes Ciara M
Institute of Nursing and Health Research, Ulster University, Jordanstown Campus, Shore Road, Newtownabbey, BT37 OQB, UK.
Centre for Cancer Research and Cell Biology, Queens University Belfast, 97 Lisburn Road, Belfast, BT9 7AE, UK.
Contrast Media Mol Imaging. 2016 Mar-Apr;11(2):122-9. doi: 10.1002/cmmi.1671. Epub 2015 Nov 8.
Paediatric cardiac catheterizations may result in the administration of substantial amounts of iodinated contrast media and ionizing radiation. The aim of this work was to investigate the effect of iodinated contrast media in combination with in vitro and in vivo X-ray radiation on lymphocyte DNA. Six concentrations of iodine (15, 17.5, 30, 35, 45, and 52.5 mg of iodine per mL blood) represented volumes of iodinated contrast media used in the clinical setting. Blood obtained from healthy volunteers was mixed with iodinated contrast media and exposed to radiation doses commonly used in paediatric cardiac catheterizations (0 mGy, 70 mGy, 140 mGy, 250 mGy and 450 mGy). Control samples contained no iodine. For in vivo experimentation, pre and post blood samples were collected from children undergoing cardiac catheterization, receiving iodine concentrations of up to 51 mg of iodine per mL blood and radiation doses of up to 400 mGy. Fluorescence microscopy was performed to assess γH2AX-foci induction, which corresponded to the number of DNA double-strand breaks. The presence of iodine in vitro resulted in significant increases of DNA double-strand breaks beyond that induced by radiation for ≥ 17.5 mg/mL iodine to blood. The in vivo effects of contrast media on children undergoing cardiac catheterization resulted in a 19% increase in DNA double-strand breaks in children receiving an average concentration of 19 mg/mL iodine to blood. A larger investigation is required to provide further information of the potential benefit of lowering the amount of iodinated contrast media received during X-ray radiation investigations.
儿科心脏导管插入术可能会使用大量的碘化造影剂并产生电离辐射。这项研究的目的是调查碘化造影剂与体外及体内X射线辐射相结合对淋巴细胞DNA的影响。六种碘浓度（每毫升血液中含15、17.5、30、35、45和52.5毫克碘）代表了临床环境中使用的碘化造影剂体积。从健康志愿者采集的血液与碘化造影剂混合，并暴露于儿科心脏导管插入术中常用的辐射剂量（0毫戈瑞、70毫戈瑞、140毫戈瑞、250毫戈瑞和450毫戈瑞）。对照样本不含碘。对于体内实验，从接受心脏导管插入术的儿童身上采集术前和术后血样，这些儿童接受的碘浓度高达每毫升血液51毫克碘，辐射剂量高达400毫戈瑞。通过荧光显微镜检查来评估γH2AX焦点诱导情况，其与DNA双链断裂的数量相对应。体外碘的存在导致DNA双链断裂显著增加，超过了辐射诱导的水平，对于每毫升血液中碘含量≥17.5毫克的情况。造影剂对接受心脏导管插入术儿童的体内影响导致，平均每毫升血液中碘含量为19毫克的儿童DNA双链断裂增加了19%。需要进行更大规模的研究，以提供关于在X射线辐射检查期间降低碘化造影剂用量潜在益处的更多信息。
