Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Microbiology and Immunology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Aging (Albany NY). 2020 Jun 2;12(11):10736-10753. doi: 10.18632/aging.103290.
As a critical factor involved in the maintenance of physiological homeostasis, the gut microbiota (GM) reportedly plays a key role in bone development. To date, the association between the GM and steroid deficiency-induced osteoporosis remains poorly understood. Forty female Sprague Dawley rats were divided into an ovariectomy (OVX) or control group. We performed 16S rRNA and metagenome sequencing, to compare diversity, taxonomic differences, and functional genes. The GM composition did not change in the control group and the number of operational taxonomic units increased significantly following ovariectomy. Alpha diversity, determined by ACE estimator, CHAO estimator, the Shannon index, and the Simpson index showed an increasing trend after ovariectomy. Samples in the OVX group were well clustered both pre- and post-ovariectomy, as demonstrated by principal coordinate 1 (PC1) and PC2. Functional genes of GM, including those involved in synthesis and metabolism of carbohydrates and nucleotides, microbial structure, and heme, as well as hemin uptake and utilization, increased at the early stage of osteoporosis. We observed that Ruminococcus flavefaciens exhibited the greatest variation in abundance among the GM and this was also associated with osteoclastic indicators and the estrobolome. Specific changes in fecal microbiota are associated with the pathogenesis of steroid deficiency-induced osteoporosis.
作为维持生理内稳态的关键因素，肠道微生物群（GM）据报道在骨骼发育中起着关键作用。迄今为止，GM 与类固醇缺乏诱导的骨质疏松症之间的关联仍知之甚少。将 40 只雌性 Sprague Dawley 大鼠分为卵巢切除术（OVX）或对照组。我们进行了 16S rRNA 和宏基因组测序，以比较多样性、分类差异和功能基因。对照组 GM 组成没有变化，而卵巢切除术后操作分类单位数量显著增加。卵巢切除术后，通过 ACE 估计值、CHAO 估计值、香农指数和辛普森指数确定的α多样性呈上升趋势。OVX 组的样本在卵巢切除前后均很好地聚类，由主坐标 1（PC1）和 PC2 证明。GM 的功能基因，包括参与碳水化合物和核苷酸合成和代谢、微生物结构和血红素以及血红素摄取和利用的基因，在骨质疏松症的早期阶段增加。我们观察到，Ruminococcus flavefaciens 在 GM 中的丰度变化最大，这也与破骨细胞指标和雌激素组有关。粪便微生物群的特定变化与类固醇缺乏诱导的骨质疏松症的发病机制有关。
