Faculty of Geoinformatics, Burapha University, Chonburi, Thailand.
Department of Microbiology, Faculty of Science, Burapha University, Chonburi, Thailand.
Trans R Soc Trop Med Hyg. 2020 Jul 1;114(7):521-530. doi: 10.1093/trstmh/traa029.
Melioidosis is an infectious disease commonly found in Thailand. This infectious disease is caused by Burkholderia pseudomallei in soil. This study aims to analyze the association between spatial soil factors and B. pseudomallei detection, as well as to map the probability of B. pseudomallei contamination based on indicator kriging in paddy soil.
Seventy-eight soil samples were collected randomly on 22 April 2018 in various paddy fields. Oxidase, Gram staining and monoclonal antibody-based latex agglutination assays were performed to confirm the presence of B. pseudomallei in soil samples. The association between B. pseudomallei detection and spatial soil factors including soil temperature, soil pH, soil texture and soil drainage were analyzed by the Mann-Whitney U test and χ2 test. Subsequently, a semivariogram model and indicator kriging were used to map the probability of B. pseudomallei contamination.
Of the 78 samples, B. pseudomallei was detected in 32 (41.03%). The presence or absence of B. pseudomallei was not significantly associated with spatial soil factors. The semivariogram model showed that the lag distance between positive B. pseudomallei samples was 90.51 m.
The empirical semivariogram and indicator kriging are an alternative option for predicting the spatial distribution of B. pseudomallei in soil.
类鼻疽是一种在泰国常见的传染病。这种传染病是由土壤中的伯克霍尔德菌引起的。本研究旨在分析空间土壤因素与假鼻疽伯克霍尔德菌检测之间的关系，并基于指示克里金在稻田土壤中绘制假鼻疽伯克霍尔德菌污染的概率图。
2018 年 4 月 22 日，在各个稻田中随机采集了 78 个土壤样本。进行氧化酶、革兰氏染色和基于单克隆抗体的乳胶凝集试验，以确认土壤样本中是否存在假鼻疽伯克霍尔德菌。采用 Mann-Whitney U 检验和 χ2 检验分析假鼻疽伯克霍尔德菌检测与土壤温度、土壤 pH 值、土壤质地和土壤排水等空间土壤因素之间的关系。随后，使用半变异函数模型和指示克里金来绘制假鼻疽伯克霍尔德菌污染的概率图。
在 78 个样本中，检测到 32 个（41.03%）含有假鼻疽伯克霍尔德菌。假鼻疽伯克霍尔德菌的存在与否与空间土壤因素无显著关联。半变异函数模型显示，阳性假鼻疽伯克霍尔德菌样本之间的滞后距离为 90.51 m。
经验半变异函数和指示克里金是预测土壤中假鼻疽伯克霍尔德菌空间分布的一种替代选择。
