Department of Developmental Pharmacology, National Organization for Drug Control and Research, Egypt.
Department of Developmental Pharmacology, National Organization for Drug Control and Research, Egypt.
Neurosci Lett. 2020 Jul 13;731:135084. doi: 10.1016/j.neulet.2020.135084. Epub 2020 May 30.
Being a critical neurodevelopmental stage that is affected by social conditions, the period of adolescence was chosen as the age of examining possible modification of alcohol neurobehavioral effects by overcrowding. Adolescent male rats (postnatal day 35±1) were subjected to overcrowding and/or injected with ethanol, 2 g/kg, 20% w/v, (i.p.) for one week. 24 h after the last dose, motor, exploratory behavior, sociability and fear responses were assessed using open field, social interaction and defensive probe burying tests, respectively. Wet brain tissue nitric oxide and reduced glutathione contents as well as monoamine levels, namely dopamine, norepinephrine and serotonin, in addition to 5-HIAA were estimated. Overcrowding increased social play and freezing time. Alcohol administration under overcrowding condition impaired sociability and interfered with active fear response. Alcohol in normal or in under overcrowding condition, impaired motor and exploratory behavior and increased anxiety. These results indicate that concomitant exposure of male adolescent rats to overcrowding and alcohol induced adverse behavioral changes.
作为一个受社会条件影响的关键神经发育阶段，青春期被选为研究过度拥挤是否可以改变酒精对神经行为影响的年龄。将青春期雄性大鼠（出生后 35±1 天）置于过度拥挤环境下，并/或腹腔注射 2g/kg、20%w/v 的乙醇，持续一周。最后一次给药后 24 小时，使用旷场试验、社会互动试验和防御性探测掩埋试验分别评估运动、探索行为、社交和恐惧反应。还测定脑组织中一氧化氮和还原型谷胱甘肽的含量以及单胺类物质（多巴胺、去甲肾上腺素和血清素）水平，此外还测定了 5-HIAA 的含量。过度拥挤增加了社会游戏和冻结时间。在过度拥挤条件下给予酒精会损害社交能力，并干扰主动恐惧反应。在正常或过度拥挤条件下给予酒精，会损害运动和探索行为并增加焦虑。这些结果表明，雄性青春期大鼠同时暴露于过度拥挤和酒精会引起不良的行为变化。
