Enhanced P450 fatty acid decarboxylase catalysis by glucose oxidase coupling and co-assembly for biofuel generation.

Cytochrome P450 OleT is a fatty acid decarboxylase that uses hydrogen peroxide (HO) to catalyze the production of terminal alkenes, which are industrially important chemicals with biofuel and synthetic applications. Despite its requirement for large turnover levels, high concentrations of HO may cause heme group degradation, diminishing enzymatic activity and limiting broad application for synthesis. Here, we report an artificial enzyme cascade composed of glucose oxidase (GOx) and OleT from Staphylococcus aureus for efficient terminal alkene production. By adjusting the ratio of GOx to OleT, the GOx-based tandem catalysis shows significantly improved product yield compared to the HO injection method. Moreover, the co-assembly of the GOx/OleT enzymes with a polymer, forming polymer-dual enzymes nanoparticles, displays improved activity compared to the free enzyme. This dual strategy provides a simple and efficient system to transform a naturally abundant feedstock to industrially important chemicals.