Graduate School of Engineering Science and Technology, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan.
Bachelor Program in Interdisciplinary Studies, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan.
Sensors (Basel). 2020 May 29;20(11):3086. doi: 10.3390/s20113086.
A novel multi-microchannel biochip fiber-optic particle plasmon resonance (FOPPR) sensor system for the simultaneous detection of multiple samples. The system integrates a novel photoelectric system, a lock-in module, and an all-in-one platform incorporating optical design and mechanical design together to improve system stability and the sensitivity of the FOPPR sensor. The multi-microchannel FOPPR biochip has been developed by constructing a multi-microchannel flow-cell composed of plastic material to monitor and analyze five samples simultaneously. The sensor system requires only 30 μL of sample for detection in each microchannel. Moreover, the total size of the multi-microchannel FOPPR sensor chip is merely 40 mm × 30 mm × 4 mm; thus, it is very compact and cost-effective. The analysis was based on calibration curves obtained from real-time sensor response data after injection of sucrose solution, streptavidin and anti-dinitrophenyl (anti-DNP) antibody of known concentrations over the chips. The results show that the multi-microchannel FOPPR sensor system not only has good reproducibility (coefficient of variation (CV) < 10%), but also excellent refractive index resolution (6.23 ± 0.10 × 10 refractive index unit (RIU)). The detection limits are 2.92 ± 0.28 × 10 g/mL (0.53 ± 0.01 nM) and 7.48 ± 0.40 × 10 g/mL (0.34 ± 0.002 nM) for streptavidin and anti-DNP antibody, respectively.
一种用于同时检测多个样本的新型多微通道生物芯片光纤粒子等离子体共振（FOPPR）传感器系统。该系统集成了一种新型光电系统、锁相模块和一个集光学设计和机械设计于一体的一体机平台，以提高系统稳定性和 FOPPR 传感器的灵敏度。多微通道 FOPPR 生物芯片通过构建由塑料材料组成的多微通道流池来开发，以同时监测和分析五个样本。传感器系统在每个微通道中仅需 30μL 的样本进行检测。此外，多微通道 FOPPR 传感器芯片的总尺寸仅为 40mm×30mm×4mm，因此非常紧凑且具有成本效益。分析基于在芯片上注入已知浓度的蔗糖溶液、链霉亲和素和抗二硝基苯（anti-DNP）抗体后的实时传感器响应数据的校准曲线进行。结果表明，多微通道 FOPPR 传感器系统不仅具有良好的重现性（变异系数（CV）<10%），而且具有出色的折射率分辨率（6.23±0.10×10 折射率单位（RIU））。链霉亲和素和抗 DNP 抗体的检测限分别为 2.92±0.28×10 g/mL（0.53±0.01 nM）和 7.48±0.40×10 g/mL（0.34±0.002 nM）。
