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通过增材制造薄网的绿色加工生产的陶瓷骨间隔物的生物学反应

Biological Responses of Ceramic Bone Spacers Produced by Green Processing of Additively Manufactured Thin Meshes.

作者信息

Minguella-Canela Joaquim, Calero Jose Antonio, Korkusuz Feza, Korkusuz Petek, Kankılıç Berna, Bilgiç Elif, De Los Santos-López M Antonia

机构信息

Centre CIM, Departament d'Enginyeria Mecànica, Universitat Politècnica de Catalunya, Av. Diagonal, 647, 08028 Barcelona, Spain.

AMES PM Tech Center, Camí de Can Ubach, 8. Pol. Ind. "Les Fallulles", 08620 Sant Vicenç dels Horts, Spain.

出版信息

Materials (Basel). 2020 May 30;13(11):2497. doi: 10.3390/ma13112497.

DOI:10.3390/ma13112497
PMID:32486136
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7321431/
Abstract

Bone spacers are exclusively used for replacing the tissue after trauma and/or diseases. Ceramic materials bring positive opportunities to enhance greater osteointegration and performance of implants, yet processing of porous geometries can be challenging. Additive Manufacturing (AM) opens opportunities to grade porosity levels in a part; however, its productivity may be low due to its batch processing approach. The paper studies the biological responses yielded by hydroxyapatite with β-TCP (tricalcium phosphate) ceramic porous bone spacers manufactured by robocasting 2-layer meshes that are rolled in green and sintered. The implants are assessed in vitro and in vivo for their compatibility. Human bone marrow mesenchymal stem cells attached, proliferated and differentiated on the bone spacers produced. Cells on the spacers presented alkaline phosphatase staining, confirming osteogenic differentiation. They also expressed bone-specific COL1A1, BGAP, BSP, and SPP1 genes. The fold change of these genes ranged between 8 to 16 folds compared to controls. When implanted into the subcutaneous tissue of rabbits, they triggered collagen fibre formation and mild fibroblastic proliferation. In conclusion, rolled AM-meshes bone spacers stimulated bone formation in vitro and were biocompatible in vivo. This technology may give the advantage to custom produce spacers at high production rates if industrially upscaled.

摘要

骨间隔物专门用于在创伤和/或疾病后替代组织。陶瓷材料为增强植入物的骨整合和性能带来了积极机遇，但多孔几何结构的加工可能具有挑战性。增材制造（AM）为在部件中分级孔隙率水平提供了机会；然而，由于其批量加工方式，其生产率可能较低。本文研究了通过对两层绿色轧制并烧结的网格进行喷射铸造制造的含β-磷酸三钙（TCP）的羟基磷灰石陶瓷多孔骨间隔物所产生的生物学反应。对植入物进行了体外和体内兼容性评估。人骨髓间充质干细胞在所生产的骨间隔物上附着、增殖并分化。间隔物上的细胞呈现碱性磷酸酶染色，证实了成骨分化。它们还表达了骨特异性COL1A1、BGAP、BSP和SPP1基因。与对照相比，这些基因的倍数变化在8至16倍之间。当植入兔的皮下组织时，它们引发了胶原纤维形成和轻度成纤维细胞增殖。总之，轧制的增材制造网格骨间隔物在体外刺激了骨形成，并且在体内具有生物相容性。如果进行工业规模扩大，这项技术可能具有以高生产率定制生产间隔物的优势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/88f263ae691d/materials-13-02497-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/1a1066440dd6/materials-13-02497-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/88f263ae691d/materials-13-02497-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/9d28d18e2013/materials-13-02497-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/4359d6d43d02/materials-13-02497-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/e9eca88660d5/materials-13-02497-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/f93a85225ea1/materials-13-02497-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff41/7321431/4b2c6bd100fb/materials-13-02497-g008.jpg
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