Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, 54000, Pakistan; Department of Oral Biology, University of Health Sciences Lahore, Khayaban-e-Jamia Punjab, Lahore, 54600, Pakistan.
Interdisciplinary Research Centre in Biomedical Materials, COMSATS University Islamabad, Lahore Campus, 54000, Pakistan.
Mater Sci Eng C Mater Biol Appl. 2020 Aug;113:110956. doi: 10.1016/j.msec.2020.110956. Epub 2020 Apr 15.
A thermo-responsive injectable bioactive glass (BAG) that has the ability to set at body temperature was prepared using pluronic F127 and hydroxypropyl methylcellulose as the carrier. The injectable composite has the advantage to fill irregular shape implantation sites and quick setting at body temperature. The structural and morphological analysis of injectable BAG before and after setting was done by using Fourier Transform Infrared spectroscopy (FTIR), and Scanning Electron Microscope (SEM). The effect of an ultrasonic scaler for a quick setting of injectable BAG was also investigated. The ultrasonic scaler sets the BAG formulation three-folds faster than at body temperature and homogenized the dispersion. The in vitro bio-adhesion was studied in the bovine tooth in both artificial saliva and deionized water for periodic time intervals, i.e., day 7, 30, 90, and 180, which confirmed the apatite layer formation. The mineral density analysis was used to differentiate the newly formed apatite with tooth apatite. In the MTT assay, the experimental material showed continuous proliferation and cell growth. This indicated that injectable hydrogel promoted cell growth, facilitated proliferation, and had no cytotoxic effect. The SEM and micro-CT results (performed after in vitro bioactivity testing) showed that the injectable BAG had the ability to regenerate dentin, hence this material has the potential to be used for dental and biomedical applications including tooth and bone regeneration in minimally invasive procedures in future.
一种具有体温响应能力的可注射生物活性玻璃（BAG），采用 Pluronic F127 和羟丙基甲基纤维素作为载体制备而成。这种可注射复合材料的优点是能够填充不规则形状的植入部位，并在体温下快速凝固。通过傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）对可注射 BAG 在凝固前后的结构和形态进行了分析。还研究了超声洁牙机对可注射 BAG 快速凝固的影响。与在体温下凝固相比，超声洁牙机将 BAG 制剂的凝固速度提高了三倍，并使分散更均匀。在牛牙中，在人工唾液和去离子水中，分别在第 7、30、90 和 180 天进行了体外生物黏附研究，证实了磷灰石层的形成。矿物密度分析用于区分新形成的磷灰石和牙磷灰石。在 MTT 测定中，实验材料显示出持续的增殖和细胞生长。这表明可注射水凝胶促进了细胞生长、增殖，并且没有细胞毒性作用。SEM 和 micro-CT 结果（在体外生物活性测试后进行）表明，可注射 BAG 具有再生牙本质的能力，因此该材料有可能在未来的微创程序中用于牙科和生物医学应用，包括牙齿和骨骼再生。
