Warsaw University of Technology, Faculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Waryńskiego 1, 00-645 Warsaw, Poland.
Warsaw University of Technology, Faculty of Chemical and Process Engineering, Department of Biotechnology and Bioprocess Engineering, Waryńskiego 1, 00-645 Warsaw, Poland.
Mater Sci Eng C Mater Biol Appl. 2020 Aug;113:110960. doi: 10.1016/j.msec.2020.110960. Epub 2020 Apr 15.
Known techniques for modification of polypropylene membranes (PPm) often require modification of the membrane in its entire volume (i.e. at the manufacturing stage), which may affect its properties. In the present work, the authors proposed a simple method for PPm hydrophilization. The process involves a two-step Fenton-type reaction, with ethylene glycol dimethacrylate (EGDMA) as a crosslinking agent and cumene hydroperoxide (CHP) as a source of free radicals. This hydrogel coating aims to enhance membrane hemocompatible and biocompatible properties. The biggest advantage of the proposed technique is the change of materials' surface properties, without interfering with its internal structure. Microscopic (SEM) and spectroscopic (FTIR-ATR) analyses confirmed the presence of hydrogel coating on PPm surfaces. Additionally, the evaluation of the surface density of the coating showed that the thickness of the coating increases with the reaction time and CHP concentration. The applied coatings significantly increase surface hydrophilicity (contact angle for PPm: 128.58° ± 0.52°, for all modified surfaces <53.31° ± 2.03°). The cytotoxicity test (XTT assay) proved biocompatibility of the PVP coating - cell viability remained above 90% for all variants tested. The modification resulted in a decrease in fibrinogen adsorption (of at least about 16%) and in a number of surface-adhered platelets. The assay evaluating the amount of secreted cell adhesion molecules (ICAM-1) showed a significant reduction (of at least about 50%) in the expression of ICAM-1 for all hydrogel-modified surfaces.
已知的聚丙稀膜（PPm）改性技术通常需要对整个膜体积（即在制造阶段）进行改性，这可能会影响其性能。在本工作中，作者提出了一种简单的 PPm 亲水改性方法。该过程涉及两步芬顿型反应，其中乙二醇二甲基丙烯酸酯（EGDMA）用作交联剂，过氧化枯烯（CHP）用作自由基源。这种水凝胶涂层旨在提高膜的血液相容性和生物相容性。所提出的技术的最大优势是改变材料的表面性质，而不干扰其内部结构。微观（SEM）和光谱（FTIR-ATR）分析证实了水凝胶涂层存在于 PPm 表面。此外，涂层表面密度的评估表明，涂层的厚度随反应时间和 CHP 浓度的增加而增加。所施加的涂层显著提高了表面亲水性（PPm 的接触角：128.58°±0.52°，所有改性表面<53.31°±2.03°）。细胞毒性试验（XTT 试验）证明了 PVP 涂层的生物相容性-所有测试变体的细胞活力保持在 90%以上。改性导致纤维蛋白原吸附减少（至少约 16%）和表面附着的血小板数量减少。评估细胞黏附分子（ICAM-1）分泌量的测定表明，所有水凝胶改性表面的 ICAM-1 表达均显著降低（至少约 50%）。
