V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2020 May;169(1):122-129. doi: 10.1007/s10517-020-04838-1. Epub 2020 Jun 2.
Light luminescent microscopy was used to study the distribution of extracellular microvesicles with PKH26-stained membranes secreted by placenta-derived mesenchymal stromal cells in the uterine tissues at different terms after injections to intact rats and after abdominal delivery (a model of cesarian section). Microvesicles migrated through the uterine tissues and were detected for at least 8 days after injection. In some cases, microvesicles were more numerous in the uterus after cesarian section modeling, which can be related to blockade of microcirculation and lymph flow due to inflammation accompanying surgical intervention. The content of microvesicles in the uterine tissues gradually declined due to macrophage phagocytosis and, probably, due to their migration into the vascular bed. Despite their size, properly stained extracellular microvesicles can be detected by light microscopy in tissues after injections.
采用荧光显微镜观察经 PKH26 染色的胎盘来源间充质基质细胞分泌的膜外微囊泡在完整大鼠子宫组织中的分布，并在注射后不同时间及剖腹产后（剖宫产模型）的子宫组织中进行检测。微囊泡通过子宫组织迁移，至少在注射后 8 天内被检测到。在某些情况下，剖宫产模型后子宫中的微囊泡数量更多，这可能与手术干预伴随的炎症引起的微循环和淋巴液流动受阻有关。由于巨噬细胞吞噬作用，以及微囊泡可能迁移到血管床中，子宫组织中的微囊泡含量逐渐减少。尽管微囊泡体积较小，但注射后组织中的光镜仍可检测到经过适当染色的外泌体。
