Abid Allah Ditta, Saeed Shafqat, Zaka Syed Muhammad, Ali Muhammad, Shahzad Muhammad Sohail, Khan Khalid Ali, Iqbal Naeem
Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan 60800, Pakistan.
Department of Plant Protection, Karachi Ministry of National Food Security, Pakistan.
Saudi J Biol Sci. 2020 Jun;27(6):1419-1422. doi: 10.1016/j.sjbs.2020.02.023. Epub 2020 Mar 10.
Hübner (Lepidoptera: Noctuidae) is serious pests of cotton and several other crops. (HaNPV) can be important alternative to synthetic insecticides for the management of . However, the efficacy of HaNPV can vary in horizontal and vertical transmission. In the current study, we evaluated the efficacy of HaNPV of a virulent strain (vertically transmitted up to six generations) and wild strains (used after isolation from the field infected larvae). Both strains were applied to the 2nd instar larvae of @ 1 × 10 polyhedral inclusion bodies (PIB)/ml. There were six replications of each strain (strains). The results indicated higher mortalities in larvae exposed to virulent strains (68.33 ± 6.07%) as compared to wild strain (45 ± 2.24%). Virulent strains killed the larvae quite faster than wild strain. The lethal time (LT) to kill 50% of the larvae by virulent strain was 7.15 days and for wild strain it was 19.47 days. The results showed that multiple passage of HaNPV through several generations enhances its efficacy to kill larvae faster. The results of this study will be helpful to manage and other related lepidopoterous pests.
棉铃虫（鳞翅目：夜蛾科）是棉花和其他几种作物的重要害虫。棉铃虫核型多角体病毒（HaNPV）可成为用于防治棉铃虫的合成杀虫剂的重要替代品。然而，HaNPV在水平和垂直传播中的效力可能会有所不同。在本研究中，我们评估了一个强毒株（垂直传播多达六代）和野生毒株（从田间感染幼虫中分离后使用）的HaNPV的效力。两种毒株均以1×10个多角体包涵体（PIB）/毫升的剂量施用于二龄幼虫。每个毒株有六个重复样本。结果表明，与野生毒株（45±2.24%）相比，暴露于强毒株的幼虫死亡率更高（68.33±6.07%）。强毒株杀死幼虫的速度比野生毒株快得多。强毒株杀死50%幼虫的致死时间（LT）为7.15天，野生毒株为19.47天。结果表明，HaNPV经过多代多次传代可提高其更快杀死幼虫的效力。本研究结果将有助于防治棉铃虫和其他相关鳞翅目害虫。
