Herniou Elisabeth A, Olszewski Julie A, Cory Jennifer S, O'Reilly David R
Department of Biological Sciences, Imperial College of Science, Technology and Medicine, London SW7 2AZ, United Kingdom.
Annu Rev Entomol. 2003;48:211-34. doi: 10.1146/annurev.ento.48.091801.112756. Epub 2001 Sep 28.
Comparative analysis of the complete genome sequences of 13 baculoviruses revealed a core set of 30 genes, 20 of which have known functions. Phylogenetic analyses of these 30 genes yielded a tree with 4 major groups: the genus Granulovirus (GVs), the group I and II lepidopteran nucleopolyhedroviruses (NPVs), and the dipteran NPV, CuniNPV. These major divisions within the family Baculoviridae were also supported by phylogenies based on gene content and gene order. Gene content mapping has revealed the patterns of gene acquisitions and losses that have taken place during baculovirus evolution, and it has highlighted the fluid nature of baculovirus genomes. The identification of shared protein phylogenetic profiles provided evidence for two putative DNA repair systems and for viral proteins specific for infection of lymantrid hosts. Examination of gene order conservation revealed a core gene cluster of four genes, helicase, lef-5, ac96, and 38K(ac98), whose relative positions are conserved in all baculovirus genomes.
对13种杆状病毒的全基因组序列进行比较分析，发现了一组由30个基因组成的核心基因集，其中20个基因具有已知功能。对这30个基因进行系统发育分析，得到一棵包含4个主要类群的树：颗粒体病毒属（GVs）、鳞翅目核型多角体病毒（NPVs）的I组和II组，以及双翅目NPV——库蚊NPV（CuniNPV）。杆状病毒科内的这些主要分类也得到了基于基因含量和基因顺序的系统发育分析的支持。基因含量图谱揭示了杆状病毒进化过程中发生的基因获得和丢失模式，并突出了杆状病毒基因组的流动性。共享蛋白质系统发育谱的鉴定为两个假定的DNA修复系统以及感染舞毒蛾宿主所特有的病毒蛋白提供了证据。对基因顺序保守性的研究揭示了一个由四个基因组成的核心基因簇，即解旋酶、lef-5、ac96和38K（ac98），它们在所有杆状病毒基因组中的相对位置都是保守的。
