Marzban Rasoul, He Qian, Liu Xiaoxia, Zhang Qingwen
Department of Entomology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
J Invertebr Pathol. 2009 Apr;101(1):71-6. doi: 10.1016/j.jip.2009.02.008. Epub 2009 Mar 6.
In this study, interactions on the mortality and debilitating effects between Cry1Ac, a toxic protein produced by Bacillus thuringiensis (Berliner) and HaCPV (Chinese strain) on first and third instars larvae of Helicoverpa armigera were evaluated in laboratory. When first instar was exposed to combination of Bt cotton leaf discs containing HaCPV (6 x 10(6), 1 x 10(7), and 3 x 10(7) PIB ml(-1)) the effect on mortality was additive, when such instar larvae exposed to combination of Cry1Ac (0.9, 2.7, or 8.1 microg g(-1)) and the same concentrations of HaCPV the effect on mortality was additive except for the combination of Cry1Ac (0.3 microg g(-1)) and HaCPV concentrations that showed synergism. When third instars of H. armigera were infected using a suspension containing both HaCPV and Cry1Ac, most combinations of them showed additive effect except for the combination of Cry1Ac (0.3 microg g(-1)) and HaCPV (3 x 10(7) PIB ml(-1)) that showed synergism. However, when they exposed to Bt cotton leaf discs and HaCPV the effect on mortality was synergism except combination of Bt cotton leaf discs and HaCPV (6 x 10(6) PIB ml(-1)) that showed additive. Most of the combinations are showed additive effect in the toxicity and in combinations of Cry1Ac at lowest and HaCPV at highest concentrations synergism is observed. Not only were larval growth and development delayed, but pupation and pupal weight also decreased when larvae were fed on artificial diet containing Cry1Ac and HaCPV or transgenic Bt cotton leaf discs specially in first instar.
在本研究中，在实验室评估了苏云金芽孢杆菌（Berliner）产生的有毒蛋白Cry1Ac与棉铃虫核型多角体病毒（中国株，HaCPV）对棉铃虫一龄和三龄幼虫死亡率及致衰弱作用的相互影响。当一龄幼虫接触含有HaCPV（6×10⁶、1×10⁷和3×10⁷PIB ml⁻¹）的Bt棉叶圆盘组合时，对死亡率的影响是相加的；当该龄期幼虫接触Cry1Ac（0.9、2.7或8.1μg g⁻¹）与相同浓度HaCPV的组合时，除Cry1Ac（0.3μg g⁻¹）与HaCPV浓度组合显示协同作用外，对死亡率的影响是相加的。当用同时含有HaCPV和Cry1Ac的悬浮液感染棉铃虫三龄幼虫时，除Cry1Ac（0.3μg g⁻¹）与HaCPV（3×10⁷PIB ml⁻¹）组合显示协同作用外，它们的大多数组合显示相加作用。然而，当它们接触Bt棉叶圆盘和HaCPV时，除Bt棉叶圆盘与HaCPV（6×10⁶PIB ml⁻¹）组合显示相加作用外，对死亡率的影响是协同作用。大多数组合在毒性方面显示相加作用，在Cry1Ac浓度最低和HaCPV浓度最高的组合中观察到协同作用。当幼虫取食含有Cry1Ac和HaCPV的人工饲料或转基因Bt棉叶圆盘时，尤其是一龄幼虫，不仅幼虫生长发育延迟，而且化蛹和蛹重也降低。
