Li Liqi, Gong Zhaoyong, Xue Piliang, Wang Dandan, Xu Meixiu, Sui Shumei, Li Liya
Department of Nephrology, Heilongjiang Academy of Traditional Chinese Medicine, Harbin 150036, Heilongjiang Province, China.
College of Life Sciences, Heilongjiang University, 74 Xuefu Road, Nangang District, Harbin City 150080, Heilongjiang Province, China.
Saudi J Biol Sci. 2020 Jun;27(6):1521-1526. doi: 10.1016/j.sjbs.2020.04.034. Epub 2020 Apr 25.
The purpose of this study was to investigate the expression of miRNA-223 and NLRP3 in IgA patients and the intervention of traditional Chinese medicine (TCM), so as to realize the basic pathological changes of IgA patients, the expression of miRNA-223 and NLRP3 in IgA patients and the changes of patients' body indexes before and after the treatment of TCM.
Firstly, according to the clinical data, patients with IgA nephropathy were divided into different groups according to their pathological changes. After that, the chemical sections and staining steps of the immune kidney were carried out. Immunohistochemical pv-9000 two-step method was used to stain it. By this method, miRNA-223 and NLRP3 genes in kidney were determined. After that, the image analysis method was used for semi quantitative experiment. Finally, the intervention of TCM was used to study the changes of indicators before and after treatment.
miRNA-223 and NLRP3 genes could be found mainly in the cytoplasm of renal tubular epithelial cells and the interstitium of monocyte in renal tissue, and there were significant differences between miRNA-223 and NLRP3 genes in the expression levels of proteinuria alone, hematuria albuminuria alone and hematuria alone. There was a positive correlation between miRNA-223 and NLRP3 expression and 24-hour urinary protein in IgA nephropathy. In addition, it also had positive correlation with MCP-1 and IL-18.
This study could provide some direction and guidance for clinical diagnosis and treatment of IgA nephropathy.
本研究旨在探讨微小RNA-223（miRNA-223）和NLRP3在IgA肾病患者中的表达情况以及中医药的干预作用，以了解IgA肾病患者的基本病理变化、miRNA-223和NLRP3在IgA肾病患者中的表达情况以及中医药治疗前后患者身体指标的变化。
首先，根据临床资料，将IgA肾病患者按病理变化分为不同组。之后，进行免疫肾的化学切片及染色步骤。采用免疫组化pv-9000两步法进行染色。通过该方法，测定肾脏中的miRNA-223和NLRP3基因。之后，采用图像分析方法进行半定量实验。最后，运用中医药进行干预，研究治疗前后指标的变化。
miRNA-223和NLRP3基因主要可在肾小管上皮细胞的细胞质及肾组织单核细胞间质中发现，单独蛋白尿、单独血尿蛋白尿及单独血尿情况下，miRNA-223和NLRP3基因的表达水平存在显著差异。IgA肾病中miRNA-223和NLRP3表达与24小时尿蛋白之间存在正相关。此外，其与单核细胞趋化蛋白-1（MCP-1）和白细胞介素-18（IL-18）也呈正相关。
本研究可为IgA肾病的临床诊断与治疗提供一定的方向和指导。
