Khanmohammadi Majid, Akhlaghi Lame, Razmjou Elham, Falak Reza, Zolfaghari Emameh Reza, Mokhtarian Kobra, Arshadi Mehdi, Tasbihi Minoo, Meamar Ahmad Reza
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Marand, Iran.
Iran J Parasitol. 2020 Jan-Mar;15(1):57-66.
Dirofilariasis is a globally distributed arthropod-borne parasitic disease of mainly canids and felids. We evaluated to extend the knowledge of morpho-molecular characteristics and outer ultrastructure of isolated from Northwest of Iran.
Overall, 67 filarial worms including 41 females and 26 males parasites were collected from the cardiovascular system of the 43 stray dogs in Meshkinshar, Ardebil Province, Northwest of Iran in 2017, and subjected to light and scanning electron microscopy (SEM) as well as carmine alum staining for morpho-molecular and identification. Molecular methods were used for confirmation of morphological findings by sequencing of Cyto-chrome c oxidase subunit I () gene.
The partial DNA sequencing of gene of adult parasites showed considerable homology and close proximity to the previously isolated from Kerman and Meshkinshahr, Iran. The lowest genetic variation and the highest intra-species variability was found in and , respectively. No similarity was identified between nucleotide sequence and species as its endosymbiont bacteria.
The SEM technique is an excellent tool for differential recognition of the parasite surface morphology and molecular techniques could differentiate and identify spp.
恶丝虫病是一种主要在犬科动物和猫科动物中传播的全球性节肢动物传播的寄生虫病。我们评估了从伊朗西北部分离出的[寄生虫名称未给出]的形态分子特征和外部超微结构，以扩展相关知识。
2017年，从伊朗西北部阿尔达比勒省梅什金沙尔的43只流浪狗的心血管系统中收集了67条丝虫，包括41只雌性和26只雄性寄生虫，并对其进行了光学显微镜和扫描电子显微镜（SEM）观察以及洋红明矾染色，以进行形态分子分析和鉴定。分子方法用于通过细胞色素c氧化酶亚基I（[基因名称未给出]）基因测序来确认形态学发现。
成年寄生虫[基因名称未给出]基因的部分DNA测序显示，与先前从伊朗克尔曼和梅什金沙尔分离出的[寄生虫名称未给出]具有相当的同源性和密切的亲缘关系。在[具体基因或区域未明确]中发现了最低的遗传变异，在[具体基因或区域未明确]中发现了最高的种内变异性。在[基因名称未给出]核苷酸序列与其作为内共生细菌的[细菌名称未给出]物种之间未发现相似性。
扫描电子显微镜技术是鉴别寄生虫表面形态的优秀工具，分子技术可以区分和鉴定[寄生虫名称未给出]物种。
