Radfar Mohammad Hossein, Fotoohi Soheila, Azizi Shahrzad, Kheirandish Reza
Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Iran J Parasitol. 2020 Jan-Mar;15(1):101-108.
is a worldwide zoonotic cestode that lives mainly in the intestine of dog as definitive host. Its larval stage infects intermediate hosts and forms hydatid cysts mainly in the liver and lungs tissues and less other organs such as brain, eye and bone. In the experimental models, inoculation of protoscoleces into the peritoneum, thoracic cavity, subcutaneous and cerebrum produces hydatid cysts. Experimental echinococcosis in the animal models provides a good opportunity for study of the parasite-host relationship, different transmission ways of infection in the intermediate hosts and effect of new drugs.
The present study was conducted in the Veterinary School, Shahid Bahonar University of Kerman, Kerman, Iran in 2018. In this study, cerebral hydatidosis was investigated in 6 female Wistar rats weighing (200±20 gr). For this purpose, protoscoleces were collected from hydatid cysts of infected sheep liver. Overall, 300 protoscolices were injected directly in the lateral ventricle by an insulin syringe through the implanted cannula.
After 4 months of inoculation, multiple thin-walled, transparent hydatid cysts were observed in the rat skull. All cysts were infertile. The cysts were localized prominently on the cerebral cortex and lesser in the parenchyma and ventricles. The cyst walls consisted of three layers consist of the outer layer (fibrous capsule), two parasitic layers and the endocyst layer (germinal layer). The cyst was surrounded by the inflammatory cells consist of lymphocytes, plasma cells and macrophages.
To the best of our knowledge, this research is the first experimental cerebral hydatidosis arisen from larval stage of in the animal model.
是一种全球性人畜共患的绦虫，主要寄生于犬的肠道内作为终末宿主。其幼虫阶段感染中间宿主，主要在肝脏和肺组织中形成包虫囊肿，在脑、眼和骨等其他器官中较少见。在实验模型中，将原头蚴接种到腹膜、胸腔、皮下和大脑中可产生包虫囊肿。动物模型中的实验性棘球蚴病为研究寄生虫与宿主的关系、中间宿主中不同的感染传播方式以及新药的效果提供了良好的机会。
本研究于2018年在伊朗克尔曼沙希德巴霍纳尔大学兽医学院进行。在本研究中，对6只体重为（200±20克）的雌性Wistar大鼠进行了脑包虫病研究。为此，从感染绵羊肝脏的包虫囊肿中收集原头蚴。总共通过植入的套管用胰岛素注射器将300个原头蚴直接注射到侧脑室。
接种4个月后，在大鼠颅骨中观察到多个薄壁、透明的包虫囊肿。所有囊肿均不育。囊肿主要位于大脑皮层，在实质和脑室中较少。囊肿壁由三层组成，外层为纤维囊，中间为两层寄生层，内层为生发层。囊肿被由淋巴细胞、浆细胞和巨噬细胞组成的炎性细胞包围。
据我们所知，本研究是在动物模型中首次由幼虫阶段引发的实验性脑包虫病。
