Immunology, Zoology and Entomology Department, Faculty of Science (for Girls), Al-Azhar University, Nasr City, Cairo, Egypt.
Parasitology, Zoology and Entomology Department, Faculty of Science (for Girls), Al-Azhar University, Nasr City, Cairo, Egypt.
Acta Parasitol. 2021 Dec;66(4):1490-1498. doi: 10.1007/s11686-021-00408-1. Epub 2021 Jun 10.
Echinococcosis is a common health problem in the Mediterranean and the Middle East, and manifests without any symptoms, even in the advanced stages.
The present study aimed to investigate the cell mediated-immunoregulatory milieu in rats' echinococcosis induced by three different viability status of Echinococcus granulosus especially in the semi-calcareous stage, which can be used as novel biomarkers to monitor disease progression and open the door to a deeper understanding of the pathways that could contribute to complementary echinococcosis therapies.
Rat infection with echinococcosis was induced by three different viable statuses of Echinococcus granulosus (G6) camel strain. During the different stages of parasitic infection, blood serum was harvested from rats containing low-, high-, and not viable (not completely transformed to the calcareous status) protoscoleces fluid. The host Th1/Th2 cytokines-mediated immune cell activation, as well as CD3/TCRE immunoregulation, and proliferation responses were investigated; especially in the semi-calcareous stage as this is the first report characterizing this stage.
Both IFN-γ and IL-6 levels significantly increased in the infected groups (P < 0.05), in addition, increased positive immunoreactions in splenic tissue for both CD3/TCRE and Ki-67 monoclonal antibodies.
E. granuloses infection-induced immune tolerance is involved in disease progression, and modulates the activation and regulation of host immune response, even in the early stages of infection, rather than the last stages of viability (semi-calcareous) is not neglected stage. This study is the first to report that the semi-calcareous stage causes a severe immunological response.
包虫病是地中海和中东地区常见的健康问题，即使在晚期也没有任何症状。
本研究旨在探讨大鼠包虫病中细胞介导的免疫调节环境，尤其是在半钙化阶段，这可作为监测疾病进展的新型生物标志物，并深入了解有助于包虫病治疗的途径。
用三种不同活力状态的细粒棘球绦虫（G6 骆驼株）诱导大鼠包虫病感染。在寄生虫感染的不同阶段，从含有低、高和非活力（未完全转化为钙化状态）原头蚴液的大鼠中采集血清。研究了宿主 Th1/Th2 细胞因子介导的免疫细胞激活以及 CD3/TCRE 免疫调节和增殖反应，特别是在半钙化阶段，因为这是首次对该阶段进行描述。
感染组 IFN-γ 和 IL-6 水平均显著升高（P<0.05），此外，脾组织中 CD3/TCRE 和 Ki-67 单克隆抗体的阳性免疫反应也增加。
细粒棘球绦虫感染诱导的免疫耐受参与疾病进展，并调节宿主免疫反应的激活和调节，即使在感染的早期阶段，而不是最后阶段（半钙化）也不容忽视。本研究首次报道半钙化阶段会引起严重的免疫反应。
