• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

局部晚期乳腺癌新辅助化疗期间对8号染色体非整倍体的CD45-/CD31+/DAPI+循环内皮细胞进行动态监测。

Dynamic monitoring of CD45-/CD31+/DAPI+ circulating endothelial cells aneuploid for chromosome 8 during neoadjuvant chemotherapy in locally advanced breast cancer.

作者信息

Ma Ge, Jiang Yi, Liang Mengdi, Li JiaYing, Wang Jingyi, Mao Xinrui, Veeramootoo Jordee Selvamanee, Xia Tiansong, Liu Xiaoan, Wang Shui

机构信息

Department of Breast Surgery, The First Affiliated Hospital with Nanjing Medical University, Nanjing, China.

The First Clinical Medical College of Nanjing Medical University, Nanjing, China.

出版信息

Ther Adv Med Oncol. 2020 May 18;12:1758835920918470. doi: 10.1177/1758835920918470. eCollection 2020.

DOI:10.1177/1758835920918470
PMID:32489429
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7238307/
Abstract

BACKGROUND

Neoadjuvant chemotherapy (NCT) is the standard treatment for patients with locally advanced breast cancer (LABC). The aim of this study was to verify this relationship, and to estimate the clinical value of aneuploid circulating endothelial cells (CECs) in LABC patients with different NCT responses.

METHODS

Breast cancer patients received an EC4-T4 NCT regimen. Peripheral blood mononuclear cells were obtained before NCT, and after the first and last NCT courses. A novel subtraction enrichment and immunostaining fluorescence hybridization (SE-iFISH) strategy was applied for detection of circulating rare cells (CRCs). CECs (CD45-/CD31+/DAPI+) and circulating tumor cells (CTCs) with different cytogenetic abnormalities related to chromosome 8 aneuploidy were analyzed in LABC patients subjected to NCT.

RESULTS

A total of 41 patients were enrolled. Firstly, CD31+/EpCAM+ aneuploid endothelial-epithelial fusion cells were observed in LABC patients. Further, aneuploid CECs in the peripheral blood showed a biphasic response during NCT, as they initially increased and then decreased, whereas a strong positive correlation was observed between aneuploid CECs and CTC numbers.

CONCLUSION

We determined that aneuploid CEC dynamics vary in patients with different response to chemotherapy. Elucidating the potential cross-talk between CTCs and aneuploid CECs may help characterize the process associated with the development of chemotherapy resistance and metastasis.

摘要

背景

新辅助化疗（NCT）是局部晚期乳腺癌（LABC）患者的标准治疗方法。本研究的目的是验证这种关系，并评估非整倍体循环内皮细胞（CECs）在不同NCT反应的LABC患者中的临床价值。

方法

乳腺癌患者接受EC4-T4 NCT方案。在NCT前、第一个和最后一个NCT疗程后采集外周血单个核细胞。采用一种新型减法富集和免疫染色荧光杂交（SE-iFISH）策略检测循环稀有细胞（CRCs）。对接受NCT的LABC患者分析与8号染色体非整倍体相关的具有不同细胞遗传学异常的CECs（CD45-/CD31+/DAPI+）和循环肿瘤细胞（CTCs）。

结果

共纳入41例患者。首先，在LABC患者中观察到CD31+/EpCAM+非整倍体内皮-上皮融合细胞。此外，外周血中的非整倍体CECs在NCT期间呈现双相反应，即最初增加然后减少，而非整倍体CECs与CTCs数量之间存在强正相关。

结论

我们确定不同化疗反应患者的非整倍体CEC动态变化不同。阐明CTCs与非整倍体CECs之间潜在的相互作用可能有助于描述与化疗耐药和转移发展相关的过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/4c006dd5908e/10.1177_1758835920918470-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/f6c251c4989e/10.1177_1758835920918470-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/1ec3ce9a9719/10.1177_1758835920918470-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/0d97bb618815/10.1177_1758835920918470-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/7d97b10889fd/10.1177_1758835920918470-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/03fb1df91f8c/10.1177_1758835920918470-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/4c006dd5908e/10.1177_1758835920918470-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/f6c251c4989e/10.1177_1758835920918470-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/1ec3ce9a9719/10.1177_1758835920918470-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/0d97bb618815/10.1177_1758835920918470-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/7d97b10889fd/10.1177_1758835920918470-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/03fb1df91f8c/10.1177_1758835920918470-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc2/7238307/4c006dd5908e/10.1177_1758835920918470-fig6.jpg

相似文献

1
Dynamic monitoring of CD45-/CD31+/DAPI+ circulating endothelial cells aneuploid for chromosome 8 during neoadjuvant chemotherapy in locally advanced breast cancer.局部晚期乳腺癌新辅助化疗期间对8号染色体非整倍体的CD45-/CD31+/DAPI+循环内皮细胞进行动态监测。
Ther Adv Med Oncol. 2020 May 18;12:1758835920918470. doi: 10.1177/1758835920918470. eCollection 2020.
2
Aneuploid CTC and CEC.非整倍体循环肿瘤细胞和循环内皮细胞。
Diagnostics (Basel). 2018 Apr 18;8(2):26. doi: 10.3390/diagnostics8020026.
3
Comprehensive in situ co-detection of aneuploid circulating endothelial and tumor cells.循环内皮细胞和肿瘤细胞的整体原位共检测。
Sci Rep. 2017 Aug 29;7(1):9789. doi: 10.1038/s41598-017-10763-7.
4
Nonhematogenic circulating aneuploid cells confer inferior prognosis and therapeutic resistance in gliomas.非血液源性循环非整倍体细胞使胶质瘤预后不良并产生治疗抵抗。
Cancer Sci. 2022 Oct;113(10):3535-3546. doi: 10.1111/cas.15516. Epub 2022 Aug 8.
5
Heterogeneous circulating tumor cells correlate with responses to neoadjuvant chemotherapy and prognosis in patients with locally advanced breast cancer.异质性循环肿瘤细胞与局部晚期乳腺癌患者新辅助化疗反应和预后相关。
Breast Cancer Res Treat. 2023 Aug;201(1):27-41. doi: 10.1007/s10549-023-06942-y. Epub 2023 Jun 13.
6
Clinical Application of Circulating Tumor Cells and Circulating Endothelial Cells in Predicting Bladder Cancer Prognosis and Neoadjuvant Chemosensitivity.循环肿瘤细胞和循环内皮细胞在预测膀胱癌预后及新辅助化疗敏感性中的临床应用
Front Oncol. 2022 Feb 3;11:802188. doi: 10.3389/fonc.2021.802188. eCollection 2021.
7
Circulating tumor cells may serve as a supplement to RECIST in neoadjuvant chemotherapy of patients with locally advanced breast cancer.循环肿瘤细胞可作为局部晚期乳腺癌患者新辅助化疗中RECIST标准的补充。
Int J Clin Oncol. 2022 May;27(5):889-898. doi: 10.1007/s10147-022-02125-9. Epub 2022 Feb 5.
8
Combined detection of aneuploid circulating tumor-derived endothelial cells and circulating tumor cells may improve diagnosis of early stage non-small-cell lung cancer.联合检测非整倍体循环肿瘤来源内皮细胞和循环肿瘤细胞可能会改善早期非小细胞肺癌的诊断。
Clin Transl Med. 2020 Jul;10(3):e128. doi: 10.1002/ctm2.128. Epub 2020 Jul 13.
9
Heterogeneity of Circulating Epithelial Cells in Breast Cancer at Single-Cell Resolution: Identifying Tumor and Hybrid Cells.单细胞分辨率下乳腺癌循环上皮细胞的异质性：鉴定肿瘤和杂交细胞。
Adv Biol (Weinh). 2023 Feb;7(2):e2200206. doi: 10.1002/adbi.202200206. Epub 2022 Nov 30.
10
Identification and Comprehensive Co-Detection of Necrotic and Viable Aneuploid Cancer Cells in Peripheral Blood.外周血中坏死和存活的非整倍体癌细胞的鉴定及综合联合检测
Cancers (Basel). 2021 Oct 12;13(20):5108. doi: 10.3390/cancers13205108.

引用本文的文献

1
Circulating Tumor Cell Detection for Therapeutic and Prognostic Roles in Breast Cancer.循环肿瘤细胞检测在乳腺癌治疗和预后中的作用
Cancer Med. 2025 Jun;14(11):e70902. doi: 10.1002/cam4.70902.
2
In situ phenotypic and karyotypic co-detection of aneuploid TCs and TECs in cytological specimens with abnormal cervical screening results.对宫颈筛查结果异常的细胞学标本中非整倍体滋养细胞和终末分化细胞进行原位表型和核型联合检测。
BMC Cancer. 2025 May 26;25(1):945. doi: 10.1186/s12885-025-14346-y.
3
Association between EPCAM upregulation and clinicopathological parameters and outcomes of breast cancer.

本文引用的文献

1
Identification of the plasma total cfDNA level before and after chemotherapy as an indicator of the neoadjuvant chemotherapy response in locally advanced breast cancer.在局部晚期乳腺癌患者中，化疗前后血浆总 cfDNA 水平的鉴定可作为新辅助化疗反应的指标。
Cancer Med. 2020 Apr;9(7):2271-2282. doi: 10.1002/cam4.2906. Epub 2020 Feb 3.
2
Epithelial-type systemic breast carcinoma cells with a restricted mesenchymal transition are a major source of metastasis.上皮型系统性乳腺癌细胞，其具有受限的间充质转化，是转移的主要来源。
Sci Adv. 2019 Jun 19;5(6):eaav4275. doi: 10.1126/sciadv.aav4275. eCollection 2019 Jun.
3
The Group B Streptococcal surface antigen I/II protein, BspC, interacts with host vimentin to promote adherence to brain endothelium and inflammation during the pathogenesis of meningitis.
EPCAM上调与乳腺癌临床病理参数及预后之间的关联
Int J Clin Exp Pathol. 2024 Nov 15;17(11):421-428. doi: 10.62347/EGXS1506. eCollection 2024.
4
Aneuploid Circulating Endothelial Cells with Prognostic Value in Locally Advanced Breast Cancer Patients After Neoadjuvant Chemotherapy.新辅助化疗后局部晚期乳腺癌患者中具有预后价值的非整倍体循环内皮细胞
Breast Cancer (Dove Med Press). 2024 Nov 12;16:761-768. doi: 10.2147/BCTT.S487336. eCollection 2024.
5
Aneuploid subtypes of circulating tumor cells and circulating tumor-derived endothelial cells predict the overall survival of advanced lung cancer.循环肿瘤细胞和循环肿瘤来源内皮细胞的非整倍体亚型可预测晚期肺癌的总生存期。
Front Oncol. 2023 May 5;13:829054. doi: 10.3389/fonc.2023.829054. eCollection 2023.
6
Detection of Circulating Tumor Cells Using the Attune NxT.使用 Attune NxT 检测循环肿瘤细胞。
Int J Mol Sci. 2022 Dec 20;24(1):21. doi: 10.3390/ijms24010021.
7
Case report: Post-therapeutic laryngeal carcinoma patient possessing a high ratio of aneuploid CTECs to CTCs rapidly developed malignancy in pancreas.病例报告：治疗后的喉癌患者，其非整倍体循环肿瘤上皮细胞（CTECs）与循环肿瘤细胞（CTCs）的比例较高，胰腺迅速发生恶变。
Front Oncol. 2022 Sep 12;12:981907. doi: 10.3389/fonc.2022.981907. eCollection 2022.
8
Liquid Biopsy as a Tool for the Diagnosis, Treatment, and Monitoring of Breast Cancer.液体活检作为乳腺癌诊断、治疗和监测的工具。
Int J Mol Sci. 2022 Sep 1;23(17):9952. doi: 10.3390/ijms23179952.
9
Circulating Tumor-Derived Endothelial Cells: An Effective Biomarker for Breast Cancer Screening and Prognosis Prediction.循环肿瘤来源的内皮细胞：一种用于乳腺癌筛查和预后预测的有效生物标志物。
J Oncol. 2022 Aug 28;2022:5247423. doi: 10.1155/2022/5247423. eCollection 2022.
10
Cellular Therapeutics for Chronic Wound Healing: Future for Regenerative Medicine.用于慢性伤口愈合的细胞疗法：再生医学的未来
Curr Drug Targets. 2022;23(16):1489-1504. doi: 10.2174/138945012309220623144620.
B 群链球菌表面抗原 I/II 蛋白 BspC 与宿主波形蛋白相互作用，促进其在脑膜炎发病机制中黏附至脑内皮细胞并引发炎症。
PLoS Pathog. 2019 Jun 10;15(6):e1007848. doi: 10.1371/journal.ppat.1007848. eCollection 2019 Jun.
4
Tumor endothelial cells as a potential target of metronomic chemotherapy.肿瘤内皮细胞作为节拍化疗的潜在靶点。
Arch Pharm Res. 2019 Jan;42(1):1-13. doi: 10.1007/s12272-018-01102-z. Epub 2019 Jan 2.
5
Detecting and phenotyping of aneuploid circulating tumor cells in patients with various malignancies.检测各种恶性肿瘤患者的非整倍体循环肿瘤细胞并进行表型分析。
Cancer Biol Ther. 2019;20(4):546-551. doi: 10.1080/15384047.2018.1538000. Epub 2018 Dec 20.
6
Circulating tumor cells with karyotyping as a novel biomarker for diagnosis and treatment of nasopharyngeal carcinoma.染色体核型分析的循环肿瘤细胞作为一种新型生物标志物用于鼻咽癌的诊断和治疗。
BMC Cancer. 2018 Nov 19;18(1):1133. doi: 10.1186/s12885-018-5034-x.
7
Contribution of Tumor Endothelial Cells in Cancer Progression.肿瘤内皮细胞在癌症进展中的作用。
Int J Mol Sci. 2018 Apr 24;19(5):1272. doi: 10.3390/ijms19051272.
8
Quantified postsurgical small cell size CTCs and EpCAM circulating tumor stem cells with cytogenetic abnormalities in hepatocellular carcinoma patients determine cancer relapse.量化术后小细胞大小循环肿瘤细胞和 EpCAM 具有细胞遗传学异常的肝癌患者循环肿瘤干细胞可确定癌症复发。
Cancer Lett. 2018 Jan 1;412:99-107. doi: 10.1016/j.canlet.2017.10.004. Epub 2017 Oct 13.
9
Comprehensive in situ co-detection of aneuploid circulating endothelial and tumor cells.循环内皮细胞和肿瘤细胞的整体原位共检测。
Sci Rep. 2017 Aug 29;7(1):9789. doi: 10.1038/s41598-017-10763-7.
10
Tumor endothelial cells accelerate tumor metastasis.肿瘤内皮细胞加速肿瘤转移。
Cancer Sci. 2017 Oct;108(10):1921-1926. doi: 10.1111/cas.13336. Epub 2017 Aug 17.