Miwa Tatsuaki, Idiris Alimjan, Kumagai Hiromichi
Developing & Planning Division, Technology Development General Division, Electronics Company, AGC Inc., (reside in AGC Techno Glass Co.), 3583-5 Kawashiri Yoshida-cho, Haibara-gun, Shizuoka 421-0302, Japan.
Biochemistry Team, Bio Science Division, Material Integration Laboratories, Technology General Division, AGC Inc., 1150 Hazawa-cho, Kanagawa-ku, Yokohama-shi, Kanagawa 221-8755, Japan.
Regen Ther. 2020 May 26;15:18-26. doi: 10.1016/j.reth.2020.04.008. eCollection 2020 Dec.
Cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) have great potential for regenerative medicine and drug discovery. In this study, we developed a novel protocol to more reproducibly and efficiently induce cardiomyocytes. A large quantity of uniformly sized spheroids were generated from hiPSCs using microfabricated vessels and induced into cardiac differentiation. In the middle of the cardiac differentiation process, spheroids were then dissociated into single cells and reaggregated into smaller spheroids using the microfabricated vessels. This reaggregation process raised and expression levels and improved the quality of cardiomyocyte population compared to that in a control group in which dissociation and reaggregation were not performed.
从人诱导多能干细胞（hiPSC）分化而来的心肌细胞在再生医学和药物发现方面具有巨大潜力。在本研究中，我们开发了一种新方案，以更可重复且高效地诱导心肌细胞。使用微加工容器从hiPSC生成大量大小均匀的球体，并诱导其进行心脏分化。在心脏分化过程中，然后将球体解离成单细胞，并使用微加工容器重新聚集形成较小的球体。与未进行解离和重新聚集的对照组相比，这种重新聚集过程提高了 和 表达水平，并改善了心肌细胞群体的质量。
