Seguret Magali, Vermersch Eva, Jouve Charlène, Hulot Jean-Sébastien
INSERM, PARCC, Université de Paris, F-75006 Paris, France.
CIC1418 and DMU CARTE, Assistance Publique Hôpitaux de Paris (AP-HP), Hôpital Européen Georges-Pompidou, F-75015 Paris, France.
Biomedicines. 2021 May 18;9(5):563. doi: 10.3390/biomedicines9050563.
Cardiac tissue engineering aims at creating contractile structures that can optimally reproduce the features of human cardiac tissue. These constructs are becoming valuable tools to model some of the cardiac functions, to set preclinical platforms for drug testing, or to alternatively be used as therapies for cardiac repair approaches. Most of the recent developments in cardiac tissue engineering have been made possible by important advances regarding the efficient generation of cardiac cells from pluripotent stem cells and the use of novel biomaterials and microfabrication methods. Different combinations of cells, biomaterials, scaffolds, and geometries are however possible, which results in different types of structures with gradual complexities and abilities to mimic the native cardiac tissue. Here, we intend to cover key aspects of tissue engineering applied to cardiology and the consequent development of cardiac organoids. This review presents various facets of the construction of human cardiac 3D constructs, from the choice of the components to their patterning, the final geometry of generated tissues, and the subsequent readouts and applications to model and treat cardiac diseases.
心脏组织工程旨在创建能够最佳再现人体心脏组织特征的收缩结构。这些构建体正成为模拟某些心脏功能、建立药物测试临床前平台或用作心脏修复方法治疗手段的有价值工具。心脏组织工程的最新进展大多得益于从多能干细胞高效生成心脏细胞以及新型生物材料和微制造方法的应用等重要进展。然而，细胞、生物材料、支架和几何形状的不同组合是可能的，这导致了不同类型的结构，其复杂性逐渐增加，且模仿天然心脏组织的能力也有所不同。在此，我们旨在涵盖应用于心脏病学的组织工程的关键方面以及由此产生的心脏类器官的发展。本综述介绍了构建人体心脏三维构建体的各个方面，从组件的选择到其图案化、生成组织的最终几何形状，以及随后用于模拟和治疗心脏病的读数和应用。
