Jahari Puteri Nur Syahzanani, Abdul Malik Nur Fatihah, Shamsir Mohd Shahir, Gilbert M Thomas P, Mohd Salleh Faezah
Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia.
Johor Biotechnology & Biodiversity Corporation (J-Biotech), Level 2, Bio-XCell Malaysia, No. 2, Jalan Bioteknologi 1, SiLC Industrial Park, 79200 Iskandar Puteri, Johor, Malaysia.
Data Brief. 2020 May 21;31:105721. doi: 10.1016/j.dib.2020.105721. eCollection 2020 Aug.
The spotted seahorse, population is exponentially decreasing globally due to habitat loss contributed by massive coastal urbanization as well as its large exploitation for Chinese herbal medicine. Genomic data would be highly useful to improve biomonitoring of seahorse populations in Malaysia via the usage of non-invasive approaches such as water environmental DNA. Here we report the first complete mitogenome of two individuals originating from Malaysia, generated using BGISEQ-500RS sequencer. The lengths of both mitogenomes are 16,529bp, consisting of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a control region. The overall base composition was 32.46% for A, 29.40% for T, 14.73% for G and 23.41% for C with AT rich features (61.86%). The gene organization of Malaysian were similar to that of most teleost species. A phylogenetic analysis of the genome against mtDNA data from other species showed that Malaysian samples clustered with and . Notably however, analysis of the data using BLASTn revealed they had 99.18% similarity to , and only 97.66% to and , which are all part of the unresolved complex. The mitogenomes are deposited in Genbank under the accession number MT221436 (HK1) and MT221436 (HK2).
由于大规模沿海城市化导致的栖息地丧失以及对其作为中药材的大量开发，全球范围内的斑点海马种群数量正在呈指数级下降。基因组数据对于通过使用水环境DNA等非侵入性方法改善马来西亚海马种群的生物监测将非常有用。在这里，我们报告了使用BGISEQ-500RS测序仪生成的来自马来西亚的两个个体的首个完整线粒体基因组。两个线粒体基因组的长度均为16,529bp，由13个蛋白质编码基因、22个转运RNA基因、两个核糖体RNA基因和一个控制区域组成。总体碱基组成为：A占32.46%，T占29.40%，G占14.73%，C占23.41%，具有富含AT的特征（61.86%）。马来西亚斑点海马的基因组织与大多数硬骨鱼类相似。对该基因组与其他物种的线粒体DNA数据进行的系统发育分析表明，马来西亚的样本与[具体物种1]和[具体物种2]聚类在一起。然而，值得注意的是，使用BLASTn对数据进行分析发现，它们与[具体物种3]的相似度为99.18%，与[具体物种4]和[具体物种5]的相似度仅为97.66%，而这几个物种都属于未解决的[具体物种复合体名称]复合体。这些线粒体基因组已存入Genbank，登录号为MT221436（HK1）和MT221436（HK2）。
