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来自马来西亚半岛的一个完整线粒体基因组的分子鉴定与系统发育分析。

Molecular identification and phylogenetic analysis of a complete mitogenome from Peninsular Malaysia.

作者信息

Jahari Puteri Nur Syahzanani, Mohd Azman Shahfiz, Munian Kaviarasu, Ahmad Ruzman Nor Hazwani, Shamsir Mohd Shahir, Richter Stine R, Gilbert M Thomas P, Mohd Salleh Faezah

机构信息

Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia.

Forest Biodiversity Division, Forest Research Institute Malaysia, Kepong, Malaysia.

出版信息

Mitochondrial DNA B Resour. 2020 Aug 26;5(3):3004-3006. doi: 10.1080/23802359.2020.1797583.

Abstract

The mitogenome of a plantain squirrel, , collected from Bukit Tarek Forest Reserve (Extension), Selangor, Malaysia was sequenced using BGISEQ-500RS technology. The 16,582 bp mitogenome consists of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. A phylogenetic and BLASTn analysis against other available datasets showed that the mitogenome matched with 99.49% similarity to a previously published mitogenome from Peninsular Malaysia. However, it also diverged by nearly 8% (92.24% match) from a second previously published mitogenome for the same species, sampled in East Kalimantan, Indonesia. This suggests a difference in landscape features between both localities might affect its genetic connectivity.

摘要

采用BGISEQ - 500RS技术对从马来西亚雪兰莪武吉塔雷克森林保护区(扩展区)采集的一只长鼻巨松鼠的线粒体基因组进行了测序。该16,582 bp的线粒体基因组由13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因和1个控制区组成。针对其他可用数据集的系统发育分析和BLASTn分析表明,该线粒体基因组与先前发表的来自马来西亚半岛的线粒体基因组相似度为99.49%。然而,它与之前发表的该物种在印度尼西亚东加里曼丹采样的第二个线粒体基因组也有近8%的差异(匹配度为92.24%)。这表明两个地区景观特征的差异可能会影响其基因连通性。

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