National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.
Department of Bioengineering, Northeastern University, Boston, United States.
Elife. 2020 Jun 3;9:e53603. doi: 10.7554/eLife.53603.
PIEZO1 and PIEZO2 are newly identified mechanosensitive ion channels that exhibit a preference for calcium in response to mechanical stimuli. In this study, we discovered the vital roles of , the sole ortholog in in regulating reproduction. A number of deletion alleles, as well as a putative gain-of-function mutant, of PEZO-1 caused a severe reduction in brood size. In vivo observations showed that oocytes undergo a variety of transit defects as they enter and exit the spermatheca during ovulation. Post-ovulation oocytes were frequently damaged during spermathecal contraction. However, the calcium signaling was not dramatically changed in the mutants during ovulation. Loss of PEZO-1 also led to an inability of self-sperm to navigate back to the spermatheca properly after being pushed out of the spermatheca during ovulation. These findings suggest that PEZO-1 acts in different reproductive tissues to promote proper ovulation and fertilization in .
PIEZO1 和 PIEZO2 是新发现的机械敏感性离子通道，对机械刺激表现出对钙的偏好。在这项研究中，我们发现了 在调节生殖中的重要作用。PEZO-1 的许多缺失等位基因，以及一个假定的功能获得性突变体，导致产卵量严重减少。体内观察表明，在排卵过程中，卵母细胞在进入和离开精囊时会经历各种转运缺陷。排卵时，精囊收缩过程中经常会损坏卵母细胞。然而，在排卵过程中，钙信号在 突变体中并没有明显改变。PEZO-1 的缺失也导致在排卵过程中被推出精囊后的自身精子无法正确导航回精囊。这些发现表明，PEZO-1 在不同的生殖组织中发挥作用，以促进 的正常排卵和受精。
