Center for Biotechnology, School of Pharmaceutical Sciences, Siksha O Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India.
Biostadt India Limited, Waluj, Aurangabad, Maharashtra, India.
J Appl Microbiol. 2020 Dec;129(6):1633-1643. doi: 10.1111/jam.14731. Epub 2020 Jul 9.
The aim of the study is to investigate the efficiency of Geotrichum candidum in the decolourization and mineralization of synthetic azo dyes.
It includes screening of enzymes from G. candidum and its optimization, followed by decolourization and mineralization studies. Decolourization was observed to be maximum in methyl orange (94·6%) followed by Congo red (85%), trypan blue (70·4%) and Eriochrome Black T (55·6%) in 48 h, suggesting the plausible degradation of the azo dyes by G. candidum. The enzyme activity study showed that DyP-type peroxidase has highest activity of 900 mU ml compared to that of laccase (405 mU ml ) and lignin peroxidase (LiP) (324 mU ml ) at optimized pH (6) and temperature (35°C). Moreover, the rate of decolourization was found to be directly proportional to the production of laccase and LiP, unlike DyP-type peroxidase. Furthermore, mineralization study demonstrated reduction in aromatic amines, showing 20% mineralization of methyl orange.
Geotrichum candidum with its enzyme system is able to efficiently decolourize and mineralize the experimental azo dyes.
The efficient decolourization and mineralization of azo dyes makes G. candidum a promising alternative in the treatment of textile effluent contaminated with azo dyes.
本研究旨在探讨异常毕赤酵母对合成偶氮染料的脱色和矿化效率。
包括从异常毕赤酵母中筛选和优化酶，然后进行脱色和矿化研究。结果表明，在 48 小时内，甲基橙（94.6%）的脱色效果最大，其次是刚果红（85%）、锥虫蓝（70.4%）和 Eriochrome Black T（55.6%），表明异常毕赤酵母可能对这些偶氮染料进行了降解。酶活性研究表明，DyP 型过氧化物酶的活性最高，为 900 mU/ml，而漆酶（405 mU/ml）和木质素过氧化物酶（LiP）（324 mU/ml）在优化的 pH（6）和温度（35°C）下的活性最高。此外，与 DyP 型过氧化物酶不同，脱色速率与漆酶和 LiP 的产量直接相关。此外，矿化研究表明芳香胺的减少，甲基橙的矿化率达到 20%。
异常毕赤酵母及其酶系统能够有效地对实验偶氮染料进行脱色和矿化。
异常毕赤酵母对偶氮染料的高效脱色和矿化作用使其成为处理偶氮染料污染纺织废水的有前途的替代方法。
