1Department of Physiology, Faculty of Medicine, University of Szeged, Szeged, Hungary.
2Department of Medical Physics and Informatics, Faculty of Medicine, University of Szeged, Szeged, Hungary.
Physiol Int. 2020 Mar;107(1):134-144. doi: 10.1556/2060.2020.00013.
Microvascular function has been assessed by determining the rhythmic oscillations in blood flow induced by the vasomotion of resistance vessels. Although laser-Doppler flowmetry (LDF) allows simple, non-invasive evaluation of this flow-motion in the cutaneous microcirculation, the temporal and spatial reproducibility of such assessments remains unclear.In the present study, we investigated cutaneous flow-motion in three consecutive years in eight skin regions using LDF in six healthy young volunteers. The characteristic flow-motion frequency was determined using fast-Fourier transformation. Additionally, in two years a more traditional measure of microvascular reactivity, postocclusive reactive hyperemia (PORH) was evoked in the forearm after transient brachial artery occlusion (1-2-3 min) induced by cuff inflation.Well-defined flow-motion was found in six regions showing significant differences in frequency: the highest flow-motion frequency was found in the frontal and temporal regions (8.0 ± 1.1 and 8.5 ± 1.0 cycles/min, cpm, respectively, mean ± SD) followed by the scapular, infraclavicular and coxal regions (7.5 ± 1.3; 6.7 ± 1.1 and 6.5 ± 1.2 cpm, respectively). The lowest, stable flow-motion was found in the posterior femoral region (5.5 ± 1.0 cpm), whereas flow-motion was detectable only sporadically in the limbs. The region-dependent flow-motion frequencies were very stable within individuals either between the body sides, or among the three measurements, only the infraclavicular region showed a small difference (114 ± 17%∗, % of value in 1st year; ∗P < 0.05). However, PORH indices differed after 2-3 min occlusions significantly in consecutive years.We report that flow-motion frequencies determined from LDF signals show both region-specificity and excellent intra-individual temporal and spatial reproducibility suggesting their usefulness for non-invasive follow-up of microvascular reactivity.
微血管功能已通过确定由阻力血管的血管舒缩运动引起的血流的节律性振荡来评估。尽管激光多普勒血流仪（LDF）允许对皮肤微循环中的这种流动运动进行简单，非侵入性的评估，但这种评估的时间和空间重现性尚不清楚。在这项研究中，我们使用 LDF 在六名健康的年轻志愿者的八个皮肤区域中连续三年研究了皮肤流动运动。使用快速傅里叶变换确定特征流动运动频率。此外，在两年中，通过充气袖带在短暂的肱动脉闭塞（1-2-3 分钟）后在前臂中诱发了后阻塞性充血反应性（PORH），这是一种更传统的微血管反应性测量方法。在六个区域中发现了定义明确的流动运动，这些区域的频率存在显着差异：额部和颞部的流动运动频率最高（分别为 8.0 ± 1.1 和 8.5 ± 1.0 个周期/分钟，cpm，平均值±SD），其次是肩胛，锁骨下和臀部区域（分别为 7.5 ± 1.3；6.7 ± 1.1 和 6.5 ± 1.2 cpm）。最低，稳定的血流运动出现在股后部（5.5 ± 1.0 cpm），而四肢仅偶尔可检测到血流运动。个体内的区域依赖性血流运动频率非常稳定，无论是在身体两侧之间，还是在三个测量值之间，只有锁骨下区域的差异很小（114 ± 17％∗，第一年的价值百分比； ∗P <0.05）。但是，连续几年的 PORH 指数在 2-3 分钟的闭塞后差异显着。我们报告说，从 LDF 信号确定的血流运动频率既具有区域特异性，又具有出色的个体内时间和空间重现性，表明它们对非侵入性微血管反应性随访有用。
