Eriksson N E, Ahlstedt S
Int Arch Allergy Appl Immunol. 1977;54(1):88-95. doi: 10.1159/000231811.
An enzyme-linked immunosorbent assay (ELISA) employing allergen adsorbed onto the internal surface of a plastic tube, and alkaline phosphatase conjugated anti-IgE was used for the determination of specific IgE antibodies to various inhalant allergens in serum samples from 255 individuals with asthma and/or allergic rhinitis. A total of 541 analyses were carried out and the results were compared with those of provocation tests, skin tests and the RAST. It was found that negative ELISA values with high probability indicated nonallergy and very high ELISA values (class greater than or equal to 5) indicated allergy. However, the proportion of positive ELISA values which did not correspond to clinical allergy was high, as were the number of intermediate, inconclusive values (class 1-2). Although significant correlations between the ELISA values and provocation test, skin test or the RAST were obtained with some allergens, it was concluded that our version of the ELISA has not advantages over currently used methods for allergy diagnosis.
采用将变应原吸附于塑料管内表面的酶联免疫吸附测定（ELISA）法，以及碱性磷酸酶偶联的抗IgE，来测定255例哮喘和/或变应性鼻炎患者血清样本中针对各种吸入性变应原的特异性IgE抗体。总共进行了541次分析，并将结果与激发试验、皮肤试验及放射性变应原吸附试验（RAST）的结果进行比较。发现ELISA值为阴性很可能表明无变应性，而ELISA值非常高（等级大于或等于5）则表明有变应性。然而，ELISA值为阳性但与临床变应性不相符的比例很高，中间的、不确定的值（1 - 2级）数量也很多。尽管ELISA值与某些变应原的激发试验、皮肤试验或RAST之间存在显著相关性，但得出的结论是，我们的ELISA版本在变应性诊断方面并不优于目前使用的方法。
