Käse Laura, Kraberg Alexandra C, Metfies Katja, Neuhaus Stefan, Sprong Pim A A, Fuchs Bernhard M, Boersma Maarten, Wiltshire Karen H
ALFRED-WEGENER-INSTITUT, HELMHOLTZ-ZENTRUM FüR POLAR- UND MEERESFORSCHUNG, BIOLOGISCHE ANSTALT HELGOLAND, 27498 HELGOLAND, Germany.
ALFRED-WEGENER-INSTITUT, HELMHOLTZ-ZENTRUM FüR POLAR- UND MEERESFORSCHUNG, 27570 BREMERHAVEN, Germany.
J Plankton Res. 2020 May;42(3):305-319. doi: 10.1093/plankt/fbaa017. Epub 2020 May 14.
The dynamics of diatoms and dinoflagellates have been monitored for many decades at the Helgoland Roads Long-Term Ecological Research site and are relatively well understood. In contrast, small-sized eukaryotic microbes and their community changes are still much more elusive, mainly due to their small size and uniform morphology, which makes them difficult to identify microscopically. By using next-generation sequencing, we wanted to shed light on the Helgoland planktonic community dynamics, including nano- and picoplankton, during a spring bloom. We took samples from March to May 2016 and sequenced the V4 region of the 18S rDNA. Our results showed that mixotrophic and heterotrophic taxa were more abundant than autotrophic diatoms. Dinoflagellates dominated the sequence assemblage, and several small-sized eukaryotic microbes like Haptophyta, Choanoflagellata, Marine Stramenopiles and Syndiniales were identified. A diverse background community including taxa from all size classes was present during the whole sampling period. Five phases with several communities were distinguished. The fastest changes in community composition took place in phase 3, while the communities from phases 1 to 5 were more similar to each other despite contrasting environmental conditions. Synergy effects of next-generation sequencing and traditional methods may be exploited in future long-term observations.
在黑尔戈兰湾航道长期生态研究站点，硅藻和甲藻的动态变化已被监测了数十年，并且人们对其有了相对较好的了解。相比之下，小型真核微生物及其群落变化仍然更加难以捉摸，主要是因为它们体积小且形态单一，这使得在显微镜下难以识别它们。通过使用新一代测序技术，我们希望揭示2016年春季水华期间黑尔戈兰湾浮游生物群落的动态变化，包括纳米浮游生物和微微型浮游生物。我们在2016年3月至5月期间采集样本，并对18S rDNA的V4区域进行了测序。我们的结果表明，混合营养型和异养型类群比自养型硅藻更为丰富。甲藻在序列组合中占主导地位，并且鉴定出了几种小型真核微生物，如定鞭藻门、领鞭毛虫纲、海洋不等鞭毛类和聚缩虫目。在整个采样期间都存在一个包括所有大小类群的多样化背景群落。区分出了五个阶段以及几个群落。群落组成变化最快的发生在第3阶段，而尽管环境条件不同，但第1阶段至第5阶段的群落彼此更为相似。在未来的长期观测中，可以利用新一代测序技术和传统方法的协同效应。
