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引用本文的文献

1
The Pneumococcal Iron Uptake Protein A (PiuA) Specifically Recognizes Tetradentate Febis- and Mono-Catechol Complexes.肺炎链球菌铁摄取蛋白 A（PiuA）特异性识别四齿体和单儿茶酚配合物。
J Mol Biol. 2020 Sep 4;432(19):5390-5410. doi: 10.1016/j.jmb.2020.08.005. Epub 2020 Aug 11.

本文引用的文献

1
Iron Acquisition by Bacterial Pathogens: Beyond Tris-Catecholate Complexes.细菌病原体的铁获取：超越三儿茶酚络合物。
Chembiochem. 2020 Jul 16;21(14):1955-1967. doi: 10.1002/cbic.201900778. Epub 2020 Apr 14.
2
Bacterial ABC transporters of iron containing compounds.含铁化合物的细菌 ABC 转运蛋白。
Res Microbiol. 2019 Nov-Dec;170(8):345-357. doi: 10.1016/j.resmic.2019.10.008. Epub 2019 Nov 1.
3
Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers.构象和底物结合蛋白的动态可塑性是 ABC 转运蛋白选择性转运的基础。
Elife. 2019 Mar 22;8:e44652. doi: 10.7554/eLife.44652.
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The human iron-proteome.人类铁蛋白组。
Metallomics. 2018 Sep 19;10(9):1223-1231. doi: 10.1039/c8mt00146d.
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NMRbox: A Resource for Biomolecular NMR Computation.NMRbox：生物分子核磁共振计算资源
Biophys J. 2017 Apr 25;112(8):1529-1534. doi: 10.1016/j.bpj.2017.03.011.
6
Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM siderophore analogues of varied linker length.与具有不同连接长度的 Fe(III)n-LICAM siderophore 类似物的周质结合蛋白 CeuE 的相互作用。
Sci Rep. 2017 Apr 6;7:45941. doi: 10.1038/srep45941.
7
Bacteria in an intense competition for iron: Key component of the Campylobacter jejuni iron uptake system scavenges enterobactin hydrolysis product.细菌在激烈的铁竞争中：空肠弯曲菌铁摄取系统的关键成分清除肠杆菌素水解产物。
Proc Natl Acad Sci U S A. 2016 May 24;113(21):5850-5. doi: 10.1073/pnas.1520829113. Epub 2016 May 9.
8
NMRFAM-SPARKY: enhanced software for biomolecular NMR spectroscopy.NMRFAM-SPARKY：用于生物分子核磁共振光谱学的增强软件。
Bioinformatics. 2015 Apr 15;31(8):1325-7. doi: 10.1093/bioinformatics/btu830. Epub 2014 Dec 12.
9
Protein structural information derived from NMR chemical shift with the neural network program TALOS-N.通过神经网络程序TALOS-N从核磁共振化学位移中获得的蛋白质结构信息。
Methods Mol Biol. 2015;1260:17-32. doi: 10.1007/978-1-4939-2239-0_2.
10
The solution structure, binding properties, and dynamics of the bacterial siderophore-binding protein FepB.细菌铁载体结合蛋白FepB的溶液结构、结合特性及动力学
J Biol Chem. 2014 Oct 17;289(42):29219-34. doi: 10.1074/jbc.M114.564021. Epub 2014 Aug 29.

肺炎链球菌 ABC 转运蛋白溶质结合蛋白 PiuA 的apo 和 holo 形式的 H、C、N 骨架共振分配。

H, C, N backbone resonance assignments of the apo and holo forms of the ABC transporter solute binding protein PiuA from Streptococcus pneumoniae.

机构信息

Department of Chemistry, Indiana University, Bloomington, Indiana, USA.

Graduate Program in Biochemistry, Indiana University, Bloomington, Indiana, USA.

出版信息

Biomol NMR Assign. 2020 Oct;14(2):233-238. doi: 10.1007/s12104-020-09952-9. Epub 2020 Jun 3.

DOI:10.1007/s12104-020-09952-9
PMID:32495035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7668159/
Abstract

Streptococcus pneumoniae is a Gram-positive human pathogen that causes millions of infections worldwide with an increasing occurrence of antibiotic resistance. Iron acquisition is essential for its survival and virulence, especially under host-imposed nutritional immunity. S. pneumoniae expresses several ATP-binding cassette (ABC) transporters to facilitate acquisition under iron limitation, including PitABCD, PiaABCD, and PiuBCDA. The substrate specificity of PiuBCDA is not fully established. Herein, we report the backbone H, C and N resonance assignments of the 31 kDa soluble, extracellular domain of the substrate binding protein PiuA in the apo form and in complex with Ga(III) and the catechol siderophore-mimic 4-LICAM. These studies provide valuable information for further functional studies of interactions with other proteins, metals, and small molecules.

摘要

肺炎链球菌是一种革兰氏阳性的人类病原体，在全球范围内导致数百万人感染，并且抗生素耐药性的发生率不断增加。铁的获取对于其生存和毒力至关重要，特别是在宿主施加的营养免疫下。肺炎链球菌表达几种 ATP 结合盒（ABC）转运蛋白来促进在缺铁条件下的获取，包括 PitABCD、PiaABCD 和 PiuBCDA。PiuBCDA 的底物特异性尚未完全确定。在此，我们报告了apo 形式和与 Ga(III)以及儿茶酚类铁载体类似物 4-LICAM 复合物中可溶性、细胞外结构域的 31 kDa 可溶性底物结合蛋白 PiuA 的 H、C 和 N 共振归属。这些研究为进一步研究与其他蛋白质、金属和小分子的相互作用提供了有价值的信息。