Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China.
J Cardiovasc Transl Res. 2020 Dec;13(6):1024-1032. doi: 10.1007/s12265-020-10027-2. Epub 2020 Jun 3.
Wall shear stress (WSS) plays a key role in maintaining glycocalyx function, gene expression, and structure. Experimental studies have discussed the relationship between the shedding of the endothelial glycocalyx (EG) and WSS. However, rare literature about how WSS affects the EG during cardiopulmonary bypass (CPB) was mentioned. This study aimed to investigate the correlation between the WSS of carotid arteries and shedding of the EG during CPB in humans. The WSS level was calculated in accordance with an equation. The plasma concentrations of heparan sulfate, syndecan-1, and nitric oxide were measured to reflect shedding of the EG at six time points. A negative correlation was observed between the peak wall shear stress (PWSS) and syndecan-1 (R = - 0.5, p < 0.01) and heparan sulfate (R = - 0.461, p < 0.01) during CPB. The WSS is closely associated with the components of glycocalyx shedding during CPB. The WSS produced by non-pulsatile flow during CPB may contribute to the degradation of EG.
壁面切应力（WSS）在维持糖萼功能、基因表达和结构方面起着关键作用。实验研究已经讨论了内皮糖萼（EG）脱落与 WSS 之间的关系。然而，关于 CPB 期间 WSS 如何影响 EG 的文献很少提及。本研究旨在探讨 CPB 期间颈动脉 WSS 与 EG 脱落之间的相关性。根据方程计算 WSS 水平。在六个时间点测量肝素硫酸、连接蛋白-1 和一氧化氮的血浆浓度，以反映 EG 的脱落。CPB 期间，峰值壁面切应力（PWSS）与连接蛋白-1（R = -0.5，p < 0.01）和肝素硫酸（R = -0.461，p < 0.01）呈负相关。WSS 与 CPB 期间糖萼脱落的成分密切相关。CPB 期间非搏动流产生的 WSS 可能导致 EG 降解。
