Liu Shuang-Shuang, Liu Qing, Li Pei, Yang Zhi-Hong, He Chun-Nian, Xiao Pei-Gen
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education Beijing 100193, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2020 May;45(9):2180-2185. doi: 10.19540/j.cnki.cjcmm.20200222.201.
Stilbenes is a class of natural polyphenols with 1,2-diphenylethylene as the skeleton structure which have structural and active diversity. However, there are fewer studies on their metabolic process, which limits the in-depth research and development of such components. An UPLC-MS/MS method simultaneously determining contents of ten stilbenes was firstly established in this study and applied to study the ten stilbenes of peony seed coats in the serum of C57 mice.Piceatannol was the internal standard, and methanol was used for protein precipitation, UPLC-MS/MS with negative ion mode was used for analysis, and the method was validated.The serum samples were collected and detected after mice being oral administered with 800 mg·kg~(-1) peony seed coat extracts for 8 weeks. The results showed that suffruticosol A, suffruticosol B, suffruticosol C, trans-ε-viniferin, cis-gnetin H, trans-suffruticosol D and trans-gnetin H were detected in serum samples, and the highest is suffruticosol A. The method is simple and quick with high specificity and sensitivity, and it is suitable for quantitative determination of ten stilbenes in the serum of mice.
芪类化合物是一类以1,2 - 二苯乙烯为骨架结构的天然多酚类化合物，具有结构和活性的多样性。然而，关于它们代谢过程的研究较少，这限制了此类成分的深入研究与开发。本研究首次建立了同时测定十种芪类化合物含量的超高效液相色谱 - 串联质谱法，并应用于研究C57小鼠血清中牡丹种皮的十种芪类化合物。以白皮杉醇为内标，甲醇用于蛋白沉淀，采用负离子模式的超高效液相色谱 - 串联质谱法进行分析，并对该方法进行了验证。小鼠口服800 mg·kg⁻¹牡丹种皮提取物8周后收集血清样本并进行检测。结果表明，在血清样本中检测到了没食子酰芍药苷、芍药苷、苯甲酰芍药苷、反式 - ε - 葡萄素、顺式 - 异叶天仙果素H、反式 - 没食子酰芍药醇D和反式 - 异叶天仙果素H，其中含量最高的是没食子酰芍药苷。该方法简便快速，具有高特异性和灵敏度，适用于小鼠血清中十种芪类化合物的定量测定。
