Liu De-Peng, Wang Yun, Wang Guo-You, Gu Xue-Zhu, Liu Tian-Liang, Zhang Cun, Wang Cheng-Yong
College of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2020 May;45(10):2382-2388. doi: 10.19540/j.cnki.cjcmm.20200221.305.
The chromaticity space parameters of the samples during the processing of Gardeniae Fructus Praeparatus(Jiaozhizi in Chinese herbal name, JZZ) were measured by the visual analyzer to analyze the color variation rule during the processing of JZZ, and the content changes of total reducing sugar, total amino acid and 5-hydroxymethylfurfural(5-HMF) related to Maillard reaction were measured. Pearson correlation analysis and linear regression analysis of the data were carried out by SPSS 24.0 software. The experimental results showed that the objective coloration of the samples in the processing of JZZ was basically consistent with the traditional subjective color judgment; the contents of total reducing sugar and total amino acids showed a decreasing trend during the processing of JZZ, and the content of 5-HMF showed an increasing trend, which was in line with Maillard reaction law. Pearson correlation analysis results showed that there was a significant correlation between the chromaticity space parameters L*(lightness value), a(red green value), b~(yellow blue value), E*ab(total color value) and the contents of total reducing sugar, total amino acid and 5-HMF(P<0.01), among which the values of L, a~, b*, E*ab were positively correlated with the contents of total reducing sugar and total amino acid, and negatively correlated with the contents of 5-HMF. The results of linear regression analysis also showed that these two were highly correlated. In this study, by establishing the correspondence relationship between the color change of JZZ processing and Maillard reactants, wecan not only provide a basis for the objective digital expression of subjective color of JZZ, but also provide a reference for explaining the processing mechanism of JZZ from a new perspective.
采用视觉分析仪测定了焦栀子加工过程中样品的色度空间参数,以分析焦栀子加工过程中的颜色变化规律,并测定了与美拉德反应相关的总还原糖、总氨基酸和5-羟甲基糠醛(5-HMF)的含量变化。运用SPSS 24.0软件对数据进行Pearson相关性分析和线性回归分析。实验结果表明,焦栀子加工过程中样品的客观色泽变化与传统主观颜色判断基本一致;焦栀子加工过程中总还原糖和总氨基酸含量呈下降趋势,5-HMF含量呈上升趋势,符合美拉德反应规律。Pearson相关性分析结果表明,色度空间参数L*(明度值)、a*(红绿值)、b*(黄蓝值)、Eab(总色差值)与总还原糖含量、总氨基酸含量和5-HMF含量之间存在显著相关性(P<0.01),其中L、a*、b*、E*ab值与总还原糖和总氨基酸含量呈正相关,与5-HMF含量呈负相关。线性回归分析结果也表明二者具有高度相关性。本研究通过建立焦栀子加工颜色变化与美拉德反应物之间的对应关系,不仅可为焦栀子主观颜色的客观数字化表达提供依据,还可为从新的角度解释焦栀子的炮制机理提供参考。