Xia Meng-Yu, Wang Yun, Zheng Ying-Hao, Song Ya-Nan, Liu Tian-Liang, Zhang Cun
School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450008, China Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2021 May;46(9):2197-2206. doi: 10.19540/j.cnki.cjcmm.20210220.303.
The chromatic values of the broken-fried and single-fried Gardeniae Fructus Praeparatus(GFP) were measured by the color analyzer to analyze the color variation rule, and the contents of 10 main components were determined by ultra-high performance liquid chromatography(UPLC). The multivariate statistical analysis, Pearson correlation analysis, and discriminant analysis were conducted to investigate the color and components of GFP samples. The experimental results revealed that L*, a, b~, and E~*ab decreased continuously during processing, and the color of samples gradually deepened. The trend and range of chromatic values during broken-frying and single-frying processes were basically identical. Gardenoside, crocin-Ⅰ(C-Ⅰ), and crocin-Ⅱ(C-Ⅱ) showed an obviously downward trend, while the contents of geniposidic acid and 5-hydroxymethylfurfural(5-HMF) increased significantly. Shanzhiside, deacetyl-asperulosidic acid methyl ester, and geniposide(G2) showed a downward trend. Scandoside methyl ester rose first and fell later. Genipin-1-O-gentiobioside(G1) went through a decrease-increase-decrease trend. The change trends of component contents during broken-frying and single-frying processes were generally consistent, but the change range was different. Among all the components, scandoside methyl ester and G1 showed obvious change. Because of different stir-frying time, the change rate of each component content in the process of broken-frying was higher than that in single-frying process. Additionally, geniposidic acid, gardenoside, scandoside methyl ester, C-Ⅰ, C-Ⅱ, and 5-HMF exhibited a higher correlation with apparent color. On the basis of above findings, the discriminant function of two frying processes was established, which could be applied to the discrimination of broken-fried and single-fried samples. This study analyzed the dynamic quality change rule of GFP during broken-frying and single-frying processes based on color-component correlation analysis, and found the two methods showed consistent change trend, yet with slight difference in the quality of samples. This study can provide data support for the processing of GFP.
采用色彩分析仪测定焦栀子和单炒栀子(炒栀子)的色度值,以分析其颜色变化规律,并用超高效液相色谱法(UPLC)测定10种主要成分的含量。通过多元统计分析、Pearson相关性分析和判别分析,研究炒栀子样品的颜色和成分。实验结果表明,L*、a*、b和Eab在加工过程中持续下降,样品颜色逐渐加深。焦炒和单炒过程中色度值的变化趋势和范围基本相同。栀子苷、西红花苷-Ⅰ(C-Ⅰ)和西红花苷-Ⅱ(C-Ⅱ)呈明显下降趋势,而京尼平苷酸和5-羟甲基糠醛(5-HMF)含量显著增加。山栀苷、去乙酰车叶草苷酸甲酯和栀子苷(G2)呈下降趋势。栀子苷甲酯先上升后下降。京尼平-1-O-龙胆二糖苷(G1)呈下降-上升-下降趋势。焦炒和单炒过程中各成分含量的变化趋势总体一致,但变化幅度不同。在所有成分中,栀子苷甲酯和G1变化明显。由于炒制时间不同,焦炒过程中各成分含量的变化率高于单炒过程。此外,京尼平苷酸、栀子苷、栀子苷甲酯、C-Ⅰ、C-Ⅱ和5-HMF与表观颜色的相关性较高。基于上述研究结果,建立了两种炒制过程的判别函数,可用于焦炒和单炒样品的判别。本研究基于颜色-成分相关性分析,分析了焦炒和单炒过程中炒栀子的动态质量变化规律,发现两种方法变化趋势一致,但样品质量略有差异。本研究可为炒栀子的加工提供数据支持。