Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Molecules. 2022 May 24;27(11):3369. doi: 10.3390/molecules27113369.
The intrinsic chemical components and sensory characteristics of Praeparatus (GFP) directly reflect its quality and subsequently, affect its clinical curative effect. However, there is little research on the correlation between the appearance traits and chemical compositions of GFP during heat processing. In this study, the major components of five typical processed decoction pieces of GFP were determined. With the deepening of processing, the contents of geniposidic acid and 5-HMF gradually increased, while the contents of deacetyl-asperulosidic acid methyl ester, gardenoside, and two pigments declined. Moreover, the electronic eye, electronic tongue, and electronic nose were applied to quantify GFP's sensory properties. It was found that the chroma values showed a downward trend during the processing of GFP. The results of odor showed that ammonia, alkenes, hydrogen, and aromatic compounds were the material base for aroma characteristics. Complex bitterness in GF was more obvious than that in other GFP processed products. Furthermore, one mathematical model was established to evaluate the correlation between the sensory characteristics and chemical composition of GFP during five different stages. A cluster analysis and neural network analysis contributed to recognizing the processing stage of GFP. This study provided an alternative method for the exterior and interior correlation-based quality evaluation of herbs.
Praeparatus (GFP) 的内在化学成分和感官特性直接反映其质量，并进而影响其临床疗效。然而，对于 GFP 在热加工过程中外观特征与化学成分之间的相关性研究甚少。本研究确定了 GFP 五种典型炮制饮片的主要成分。随着炮制程度的加深，獐牙菜苦苷和 5-HMF 的含量逐渐增加，而獐牙菜苦苷甲酯、龙胆苦苷和两种色素的含量则下降。此外，还应用电子眼、电子舌和电子鼻来定量 GFP 的感官特性。研究发现，在 GFP 的炮制过程中，色差值呈下降趋势。气味结果表明，氨、烯烃、氢气和芳香族化合物是香气特征的物质基础。GF 中的复杂苦味比其他 GFP 加工产品中的苦味更明显。此外，还建立了一个数学模型来评估 GFP 在五个不同阶段的感官特性和化学成分之间的相关性。聚类分析和神经网络分析有助于识别 GFP 的炮制阶段。本研究为基于内外相关性的草药质量评价提供了一种替代方法。
