Department of Nephrology, the Traditional Chinese Medicine Hospital Affiliated with Southwest Medical University, Luzhou, China.
J Int Med Res. 2020 Jun;48(6):300060520926033. doi: 10.1177/0300060520926033.
In this case-control study, we retrospectively analyzed the intestinal flora compositions of patients with early-stage chronic kidney disease (CKD).
Forty-seven patients with early CKD who were treated at the Traditional Chinese Medicine Hospital between March and October 2018 were enrolled, and 150 healthy volunteers were enrolled in the healthy control group. Fresh stool samples were collected. The V3-V4 region of the bacterial 16S rRNA was amplified via PCR. Biterminal sequencing was performed using the Illumina MiSeq platform. The flora compositions were compared between the two groups.
The Chao1 and Shannon indices showed significantly lower intestinal flora diversity and abundances in the CKD group than in the healthy controls. Beta diversity analysis revealed notable differences in the intestinal flora compositions between the groups. At the phylum level, Actinobacteria and Proteobacteria abundances were significantly higher in the CKD group. Thirty-one species differed significantly between both groups, among which, differences in and displayed the highest diagnostic values for distinguishing CKD patients from healthy controls.
Intestinal flora compositions are altered in early-stage CKD patients among the Han population in southwestern China.
在这项病例对照研究中，我们回顾性分析了早期慢性肾脏病（CKD）患者的肠道菌群组成。
2018 年 3 月至 10 月期间，我们招募了在中医院接受治疗的 47 例早期 CKD 患者作为病例组，并招募了 150 名健康志愿者作为健康对照组。采集新鲜粪便样本。采用 PCR 扩增细菌 16S rRNA 的 V3-V4 区。使用 Illumina MiSeq 平台进行双端测序。比较两组之间的菌群组成。
CKD 组的 Chao1 和 Shannon 指数均显示肠道菌群多样性和丰度显著低于健康对照组。β多样性分析显示两组之间的肠道菌群组成存在显著差异。在门水平上，CKD 组中放线菌和变形菌的丰度显著较高。两组之间有 31 个种存在显著差异，其中 和 的差异显示出区分 CKD 患者和健康对照者的最高诊断价值。
在中国西南部汉族人群中，早期 CKD 患者的肠道菌群组成发生了改变。
