Department of Nuclear Medicine, Cancer Hospital of China Medical University, Shenyang, China.
Eur Rev Med Pharmacol Sci. 2020 May;24(10):5504-5511. doi: 10.26355/eurrev_202005_21335.
The purpose of this study was to detect the expression of long non-coding ribonucleic acid 00163 (LINC00163) in human papillary thyroid cancer (PTC), and to observe the influence of downregulated LINC00163 on the proliferative and metastatic capacities of human PTC cells.
Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) assay was applied to measure the expression level of LINC00163 in PTC tissues and para-carcinoma tissues, as well as that in normal human thyroid cells (Nthy-ori3-1) and PTC cells. After the expression of LINC00163 in PTC cells was interfered, qRT-PCR assay was performed to determine the interference efficiency, and colony formation and Cell Counting Kit-8 (CCK-8) assays were conducted to study the impacts of small interfering (si)-LINC00163 on the proliferative capacity of PTC cells. Moreover, wound healing and transwell assays were adopted to investigate the changes in the migratory and invasive abilities of PTC cells after the interference in the expression of LINC00163 in PTC cells. Finally, the changes in expressions of molecular markers in downstream signaling pathways after interference in LINC00163 expression were examined via Western blotting assay.
In 51 cases of PTC tissues and corresponding para-carcinoma tissues, 41 cases exhibited an up-regulated expression of LINC00163, and qRT-PCR results indicated that PTC cells also had an up-regulated expression of LINC00163 compared with normal human thyroid cells. After the expression of LINC00163 in PTC cells was interfered, the results of colony formation and CCK-8 assays manifested that the proliferative capacity of the cells declined. It was also shown in wound-healing and transwell assay results that the migratory and invasive abilities of the cells were weakened. In addition, the results of Western blotting assay revealed expression changes in the molecular markers of epithelial-mesenchymal transition (EMT).
The expression of LINC00163 in NSCLC tissues and cells is upregulated, and highly expressed LINC00163 can promote PTC cell proliferation and metastasis by regulating the EMT.
本研究旨在检测长链非编码核糖核酸 00163（LINC00163）在人甲状腺乳头状癌（PTC）中的表达，并观察下调 LINC00163 对人 PTC 细胞增殖和转移能力的影响。
采用实时定量逆转录聚合酶链反应（qRT-PCR）检测 PTC 组织和癌旁组织、正常甲状腺细胞（Nthy-ori3-1）和 PTC 细胞中 LINC00163 的表达水平。干扰 PTC 细胞中 LINC00163 的表达后，通过 qRT-PCR 检测干扰效率，细胞集落形成和细胞计数试剂盒-8（CCK-8）检测 si-LINC00163 对 PTC 细胞增殖能力的影响。此外，采用划痕愈合和 Transwell 实验研究干扰 PTC 细胞中 LINC00163 表达后 PTC 细胞迁移和侵袭能力的变化。最后，通过 Western blot 检测干扰 LINC00163 表达后下游信号通路中分子标志物的表达变化。
在 51 例 PTC 组织和相应癌旁组织中，41 例 LINC00163 表达上调，qRT-PCR 结果显示 PTC 细胞与正常甲状腺细胞相比也呈 LINC00163 表达上调。干扰 PTC 细胞中 LINC00163 的表达后，集落形成和 CCK-8 检测结果表明细胞增殖能力下降。划痕愈合和 Transwell 实验结果也表明细胞迁移和侵袭能力减弱。此外，Western blot 检测结果显示上皮间质转化（EMT）分子标志物的表达发生变化。
LINC00163 在 NSCLC 组织和细胞中的表达上调，高表达的 LINC00163 可通过调节 EMT 促进 PTC 细胞的增殖和转移。
