Department of Chemistry, University of Leicester, University Road, LE1 7RH, Leicester, UK.
Analyst. 2020 Jun 15;145(12):4224-4232. doi: 10.1039/d0an00419g.
A novel molecularly imprinted polymer nanoparticle-based assay (MINA) performed in magnetic microplates was developed as an improved high-quality alternative to existing antibody-based immunoassays. MINA is a generic technology that can be adapted for biomarker detection in biological samples. Herein, we demonstrate the applicability of the MINA assay for the detection of leukotrienes and insulin in biological samples. MINA, used in a competition format, has allowed the detection of LTE4 in urine in a concentration range from 0.45 to 364 pM, with a LOD of 0.73 pM. MINA, used in a competition format, has allowed the detection of insulin in plasma in a concentration range from 25 to 2500 pM, with a LOD of 27 pM. This assay has shown comparable performance for LTE4 and insulin detection to existing chromatographic techniques (LC-MS/MS) and immunoassays in clinically relevant concentrations. The main advantages of this assay are the efficient and low cost fabrication, preparation of synthetic binders without the use of animals, and fewer steps used in the assay protocol as compared to traditional immunoassays.
一种新型的基于分子印迹聚合物纳米颗粒的分析方法（MINA）在磁性微孔板上进行，作为现有抗体免疫分析的改进的高质量替代方法。MINA 是一种通用技术，可以适应生物样品中生物标志物的检测。在此，我们证明了 MINA 分析方法在生物样品中检测白三烯和胰岛素的适用性。MINA 以竞争格式使用，允许在 0.45 到 364 pM 的浓度范围内检测尿液中的 LTE4，LOD 为 0.73 pM。MINA 以竞争格式使用，允许在 25 到 2500 pM 的浓度范围内检测血浆中的胰岛素，LOD 为 27 pM。与临床相关浓度下的现有色谱技术（LC-MS/MS）和免疫分析相比，该分析方法在检测 LTE4 和胰岛素方面具有相当的性能。该分析方法的主要优点是高效、低成本的制造，在不使用动物的情况下制备合成结合物，以及与传统免疫分析相比，分析方法中使用的步骤更少。
